GelRed
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IUPAC name
5,5'-(6,22-dioxo-11,14,17-trioxa-7,21-diazaheptacosane-1,27-diyl)bis(3,8-diamino-6-phenylphenanthridin-5-ium) iodide
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Other names | |
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3D model (JSmol)
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PubChem CID
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CompTox Dashboard (EPA)
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Properties | |
C60H72I2N8O5 | |
Molar mass | 1239.07 g/mol |
Hazards | |
NFPA 704 (fire diamond) | |
Flash point | > 100 °C (212 °F; 373 K) |
Safety data sheet (SDS) | 10,000X in water, Biotium Inc. |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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GelRed is an intercalating nucleic acid stain used in molecular genetics for agarose gel DNA electrophoresis. GelRed structurally consists of two ethidium subunits that are bridged by a linear oxygenated spacer.[1][2]
Its fluorophore, and therefore its optical properties, are essentially identical to those of ethidium bromide. When exposed to ultraviolet light, it will fluoresce with an orange color that strongly intensifies after binding to DNA.[3] The substance is marketed as a less toxic and more sensitive alternative to ethidium bromide.[3] GelRed is sold as a solution in anhydrous DMSO or ultrapurified water.[3]
See also
References
- ^ a b c US application 2010323453, Mao, Fei & Leung, Wai-Yee, "Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology"
- ^ GelRed & GelGreen (PDF), Biotium Inc., August 21, 2012, retrieved December 4, 2012
- ^ a b c GelRed and GelGreen: Environmentally safe and ultra-sensitive nucleic acid gel stains for replacing EtBr, Biotium Inc., retrieved December 4, 2012