HIS-selective medium

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HIS-selective medium is a type cell culture medium that lacks the amino acid histidine. It can be used with bacteria reliant on the expression of a gene encoding proteins involved in histidine expression in order to survive.[1] Only bacteria expressing such genes (such as hisB in Escherichia coli and HIS3 in Saccharomyces cerevisiae) will survive on these media.

References

  1. ^ Joung, J. K., E. I. Ramm and C. O. Pabo (2000). "A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions." Proc Natl Acad Sci U S A 97(13): 7382-7.


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