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Newbler

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Newbler
Developer(s)454 Life Sciences
Stable release
v2.7 / February 28, 2012; 12 years ago (2012-02-28)
Operating systemLinux i386/x86_64
TypeBioinformatics
LicenseCommercial

Newbler is a software package for de novo DNA sequence assembly. It is designed specifically for assembling sequence data generated by the 454 GS-series of pyrosequencing platforms sold by 454 Life Sciences, a Roche Diagnostics company.[1]

Usage

Newbler can run via a Java GUI (gsAssembler) or the command line (runAssembly). It works natively with the .SFF data output by the sequencer, but is also able to accept FASTA files, containing nucleotide sequences, with or without quality information, and FASTQ files. It will use older Sanger sequence data if appropriately formatted to aid in assembly and scaffolding.

See also

References

  1. ^ Genivaldo, GZ; Silva, Bas E; Dutilh, David; Matthews, Keri; Elkins, Robert; Schmieder, Elizabeth A; Dinsdale, Robert A Edwards (2013). "Combining de novo and reference-guided assembly with scaffold_builder". Source Code Biomed Central. 8 (23): 23. doi:10.1186/1751-0473-8-23. PMC 4177539. PMID 24267787.{{cite journal}}: CS1 maint: unflagged free DOI (link)