The complete mitochondrial protein translocase complex includes at least 19 proteins: several chaperones, four proteins of the outer membrane translocase (Tom) import receptor, five proteins of the Tom channel complex, five proteins of the inner membrane translocase (Tim) and three "motor" proteins.
Protein targeting to the mitochondria
There are various mitochondrial import pathways that exist to facilitate the import of precursor proteins to their destined mitochondrial subcompartments. HSP90 aids the delivery of the mitochondrial preprotein to the TOM complex in an ATP-dependent process.[3] Many precursor proteins (those that are destined for the matrix) contain amino-terminal presequences that carry information required for the targeting of proteins to the mitochondrial matrix[4] These matrix targeting signals generally contain 10-80 amino acid residues that take on the conformation of an amphipathic-α helix[5] and contain one positive and hydrophobic face. Once the precursor reaches the matrix, the presequence is typically cleaved off by the matrix processing peptidase.[6] Proteins targeted to other sub-compartments of the mitochondria such as the intermembrane space and inner mitochondrial membrane, contain internal targeting signals, these signals have an indefinable nature and are inconsistent in their pattern. Proteins targeted to the outer membrane also contain internal targeting signals, not all of which have been identified, and include proteins that take on a β-barrel structure,[7] such as Tom40. Some proteins however, that are targeted to the outer mitochondrial membrane contain a hydrophobic tail domain that anchors the protein to the membrane.[8]
Members of the complex
The translocase of the outer membrane (TOM) forms a complex made of Tom70, Tom22, and Tom20, along with Tom40, Tom7, Tom6, and Tom5. Tom20 and Tom22 are preprotein receptors, which are responsible for recognition of the cleavable presequence possessed by mitochondrial-targeted proteins.[9] Tom70 is also a preprotein receptor and may recognise some cleavable presequence proteins, however it is mainly responsible for the recognition of non-cleavable preproteins and acts as a point for chaperone binding.[6][10] Tom22 is anchored to the outer membrane by a single transmembrane segment and also plays a role in stabilizing the TOM complex.[11] Tom40 is the core element of the translocase complex and complexes with Tom22 with a mass of approximately 350k Daltons.[12] It forms the central protein-conducting channel with a diameter of approximately 2.5 nm.[12] The human Tom22 is approximately 15.5k Daltons and complexes with Tom20.[13] The N-terminal end of Tom22 extends into the cytosol and is involved in preprotein binding.[13]
^Seki N, Moczko M, Nagase T, et al. (1996). "A human homolog of the mitochondrial protein import receptor Mom19 can assemble with the yeast mitochondrial receptor complex". FEBS Lett. 375 (3): 307–10. doi:10.1016/0014-5793(95)01229-8. PMID7498524.
