As a little girl, she dreamed of becoming an actress but was so shy she often couldn't even answer the telephone. She studied acting at the Centro de Estudios y Formación Actoral and got her first job, Chiquititas in 1998. Serradilla is of Spanish descent with roots in Madrid. In 2012, she debuted in her first English language film, Hidden Moon for which she won the Silver Goddess "Best Actress" Award. Her theatre credits include Sin Cura and Blackbird.
As of 2014 Serradilla is best known as "La Madrina" ("The Godmother") Griselda Blanco from the popular TV series La viuda negra (2014), series which covers the life of the cocaine queen Griselda Blanco.