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Autolysis may refer to:
- Autolysis (biology), the destruction (or lysis) of a cell by its own enzymes
- Autolysis (chemistry), the production of a substance which catalyzes a chemical reaction it was made in, or catalyzes its own transformation into another compound
- Autolysis (wine), the complex chemical reactions that take place when a wine spends time in contact with lees after fermentation
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