Base calling

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Base calling is the process of assigning bases (nucleobases) to chromatogram peaks. One computer program for accomplishing this job is Phred base-calling, which is a widely used basecalling software program by both academic and commercial DNA sequencing laboratories because of its high base calling accuracy.[1]


  1. ^ Richterich P. (1998): Estimation of errors in "raw" DNA sequences: a validation study. Genome Res. 8(3):251–259. PMID 9521928