CCDC186
C10orf118 is a protein that in humans is encoded by the C10orf118 gene [2] The C10orf118 (CCDC186) gene is also known as the CTCL-tumor associated antigen with accession number NM_018017.[3]
Gene
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Location
C10orf118 has the chromosome location of 10q25.3 and is 53,750 bases in size oriented on the minus strand. PSORTII Protein k-NN Prediction indicated that C10orf118 is 65.2% nuclear, 17.4% cytosolic, 8.7% mitochondrial, 4.3% vesicles of secretory system, and 4.3% endoplasmic reticulum.[4]
Expression
Analysis of gene expression in humans and other species indicates C10orf118 (CCDC186) is ubiquitously expressed in all tissue types at varying developmental stages. An EST profile from NCBI displayed the greatest expression in bone marrow, kidneys, and the prostate cell lines. Breakdown by health state indicates high expression of C10orf118 in bladder carcinoma and prostate cancer.[5]
Homology
C10orf118 is conserved in many species. Orthologous sequences of C10orf118 were not found to be in bacteria, archea, protist, or plants. C10orf118 has no human paralogs.
Protein
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General Properties
The protein of CCDC186 (NP_060487) is 898 amino acids in length. The predicted molecular weight is 103.7kdal and the isoelectric point is predicted to be 5.92.[6]
Composition
A serine rich region is observed in amino acids 710-747. A compositional analysis revealed that C10orf118 is Proline (1.1%) poor and Glutamic acid (14.1%) and Lysine (12.0%) rich.[6]
Conservation
The date of divergence for the orthologous sequences highly correlates with the sequences similarity in that the percent identity decreases as you go back in time. Closely related orthologs include mammals and birds and moderately related orthologs include other vertebrates such as fish, reptiles, and amphibians. Distantly related orthologous sequences were primarily observed in invertebrates.[6][7]
Post Translational Modification
C10orf118 is predicted to undergo multiple posttranslational modifications including predicted O-beta-GlcNAc attachment, phosphorylation, a nuclear export signal, glycation of lysines, GlcNAc O-glycosylation, N-glycosylation, and NetCorona sites.[8]
Interacting Proteins
C10orf118 protein was found to interact with proteins PLEKAH5, Exra, GAMMAHV.ORF23, and SMAD3.[9][10]
References
- ^ http://ibs.biocuckoo.org/online.php
- ^ Oduru, S; Campbell, JL; Karri, S; Hendry, WJ; Khan, SA; Williams, SC (Jul 2003). "Gene discovery in the hamster: a comparative genomics approach for gene annotation by sequencing of hamster testis cDNAs". BMC Genomics. 4: 22. doi:10.1186/1471-2164-4-22. PMC 161800. PMID 12783626.
{{cite journal}}: CS1 maint: unflagged free DOI (link) - ^ "Entrez Gene: C10orf118 chromosome 10 open reading frame 118".
- ^ "PSORT II Prediction". psort.hgc.jp. Retrieved 2016-04-24.
- ^ "Home - UniGene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2016-04-24.
- ^ a b c "SDSC Biology Workbench". seqtool.sdsc.edu. Retrieved 2016-04-24.
- ^ "BLAST: Basic Local Alignment Search Tool". blast.ncbi.nlm.nih.gov. Retrieved 2016-04-24.
- ^ "Welcome to CBS". www.cbs.dtu.dk. Retrieved 2016-04-24.
- ^ "* in Literature citations". www.uniprot.org. Retrieved 2016-04-24.
- ^ http://www.uniprot.org/uniprot/Q7Z3E2
- ^ "PHYRE2". www.sbg.bio.ic.ac.uk/phyre2. Retrieved 2016-02-05.
Further reading
- Hartley, JL; Temple, GF; Brasch, MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
- Hartmann, TB; Thiel, D; Dummer, R; et al. (2004). "SEREX identification of new tumour-associated antigens in cutaneous T-cell lymphoma". Br. J. Dermatol. 150 (2): 252–8. doi:10.1111/j.1365-2133.2004.05651.x. PMID 14996095.
- Wiemann, S; Arlt, D; Huber, W; et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
- Mehrle, A; Rosenfelder, H; Schupp, I; et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.
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