Casamino acid

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Casamino acids are a mixture of amino acids and some very small peptides obtained from acid hydrolysis of casein [1] It is typically used in microbial growth media. It has all the essential amino acids except tryptophan which becomes almost destroyed when digested with sulfuric or hydrochloric acid.[1]

Casamino acids are similar to tryptone, the latter differing by being an incomplete enzymatic hydrolysis with some oligopeptides present, while casamino acids are predominantly free amino acids.

Uses[edit]

Casamino acids are either found in the Daptacel brand DTaP vaccine or used in its manufacture.[2] Casamino Acids is a hydrochloric acid hydrolysate of casein. Supplies a completely hydrolyzed protein nitrogen source. It contains a small amount of cystine. Tryptophan and vitamins are destroyed by the acid treatment. The remaining amino acids (in varying amounts) are a source of nutrients for various microorganisms. Amino acids are highly soluble and suitable for use in tissue culture. Salt content is typically 30-40%.

Appearance: White to light tan, homogenous, free flowing powder Solubility (2%) Colourless to light yellow, clear in solution

References[edit]

  1. ^ a b Mueller, J. Howard; Johnson, Everett R (1 Jan 1941). "Acid Hydrolysates of Casein to Replace Peptone in the Preparation of Bacteriological Media". The Journal of Immunology. 40 (1): 33–38. ISSN 1550-6606. 
  2. ^ http://www.cdc.gov/vaccines/pubs/pinkbook/downloads/appendices/B/excipient-table-2.pdf