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The Charleston Subdivision is a railroad line owned by CSX Transportation in the U.S. states of South Carolina and Georgia. The line from Florence, South Carolina to Savannah, Georgia for a total of 195.8 miles. At its north end it continues south from the South End Subdivision and at its south end it continues south as the Savannah Subdivision of the Jacksonville Division.
The line includes two original railroad lines such as the Charleston and Savannah Railway and the Northeaster Railroad of South Carolina, both of which were acquired by the Atlantic Coast Line Railroad in 1902 and 1893 respectively.
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