D65 may refer to:
- Illuminant D65, a commonly used standard illuminant defined by the International Commission on Illumination
- Greek destroyer Nearchos (D65)
- HMS Codrington (D65), a 1930 A-class destroyer of the Royal Navy
- HMS St. James (D65), a 1946 Battle-class destroyer of the Royal Navy
- SPS Blas de Lezo (D65), a Spanish Navy ship
- the former designation for the Slovakian R1 expressway
- D 65 road (United Arab Emirates) (Al Manara Road), a road passing in Umm Suqeim
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