Daisy chaining DNA

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Daisy chaining DNA is the process of when DNA undergoing PCR amplification becomes tangled and forms a 'daisy chain.' During PCR, primers or dNTP's will eventually be used up and limit further reactions. The depletion of primers causes daisy chaining; since the denaturing and annealing processes will still continue without primers, the single-stranded DNA molecules will reanneal to themselves. However, this reannealing does not always occur with another complementary strand. It is this imperfect match up that causes 'tangles'. These tangles look like a daisy chain.[1]

References[edit]

  1. ^ KapaBiosystems. "High-Throughput NGS Library Preparation Technical Guide". Kapa Biosystems. Retrieved 23 September 2015.

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