File:Explosive-cell-lysis-as-a-mechanism-for-the-biogenesis-of-bacterial-membrane-vesicles-and-biofilms-ncomms11220-s5.ogv

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Explosive-cell-lysis-as-a-mechanism-for-the-biogenesis-of-bacterial-membrane-vesicles-and-biofilms-ncomms11220-s5.ogv(Ogg Theora video file, length 7.5 s, 640 × 320 pixels, 444 kbps, file size: 406 KB)

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English: Supplementary Movie 3 Round cells are viable until explosive cell lysis. A P. aeruginosa PAK interstitial biofilm cultured on media containing the cell impermeant DNA stain EtHD-2 was imaged using phase contrast (left panel) and fluorescence (right panel) microscopy (Nikon Ti). All cells, including two round cells, exclude EtHD-2 indicating they have intact membranes. One round cell explodes and eDNA (red) is then observed. The remaining round cell continues to exclude the EtHD-2. Time is shown in minutes and seconds.
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Source Video file from Turnbull L, Toyofuku M, Hynen A, Kurosawa M, Pessi G, Petty N, Osvath S, Cárcamo-Oyarce G, Gloag E, Shimoni R, Omasits U, Ito S, Yap X, Monahan L, Cavaliere R, Ahrens C, Charles I, Nomura N, Eberl L, Whitchurch C (2016). "Explosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms". Nature Communications. DOI:10.1038/ncomms11220. PMID 27075392. PMC: 4834629.
Author Turnbull L, Toyofuku M, Hynen A, Kurosawa M, Pessi G, Petty N, Osvath S, Cárcamo-Oyarce G, Gloag E, Shimoni R, Omasits U, Ito S, Yap X, Monahan L, Cavaliere R, Ahrens C, Charles I, Nomura N, Eberl L, Whitchurch C
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14 April 2016

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current08:00, 28 October 20167.5 s, 640 × 320 (406 KB)Open Access Media Importer BotAutomatically uploaded media file from Open Access source. Please report problems or suggestions here.
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