File:Phage display.png
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Summary
| Description |
English: Phage display cycle. 1) fusion proteins for a viral coat protein + the gene to be evolved (typically an antibody fragment) are expressed in bacteriophage. 2) the library of phage are washed over an immobilised target. 3) the remaining high-affinity binders are used to infect bacteria. 4) the genes encoding the high-affinity binders are isolated. 5) those genes may have random mutations introduced and used to perform another round of evolution. The selection and amplification steps can be performed multiple times at greater stringency to isolate higher-affinity binders.
Français : traduction à améliorer : Cycle d'évolution. 1) Protéines de fusion pour une protéine virale d'enveloppe + le gène devant évoluer (typiquement un fragment d'anticorps) sont exprimées dans le bactériophage. 2) La banque de phages est lavées sur une cible immobilisée. 3) les liants à haute affinité restants sont utilisés pour infecter les bactéries. 4) les gènes codant pour les liants à haute affinité sont isolés. 5) ces gènes peuvent provoquer des mutations aléatoires introduites et utilisées pour effectuer un autre cycle d'évolution. Les étapes de sélection et d’amplification peuvent être effectuées plusieurs fois avec une plus grande rigueur pour isoler des liants d’affinité plus élevée. |
| Date | |
| Source | Own work |
| Author | Thomas Shafee |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 07:53, 22 May 2019 | | 2,826 × 1,798 (612 KB) | Evolution and evolvability | minor optimisation of label positions |
| 05:29, 22 May 2019 |  | 2,901 × 1,798 (585 KB) | Evolution and evolvability | User created page with UploadWizard |
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