English: Electropherogram showing elution profiles (corrected baselines) of a 200 kD Cd cofactor-containing protein from plant cytosol using PAGE gels polymerized for 66, 69, 69, or 93 hours, respectively. Detection method: Graphite Furnace Atomic Absorption Spectrometry.
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Denatured metalloproteins (66 h / 93 h) are characterized by conformational changes or the dissociation of metal cofactors, which leads to a change in charge ratios, and thus, the pKa and pI values.
{{Information |Description={{en|1=Electropherogram showing elution profiles (corrected baselines) of a 200 kD Cd cofactor-containing protein from plant cytosol using PAGE gels polymerized for 66, 69, 69, or 93 hours, respectively. Detection method: Graphi