Haemagglutination activity domain

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haemagglutination activity domain
PDB 1rwr EBI.jpg
crystal structure of filamentous hemagglutinin secretion domain
Identifiers
Symbol Haemagg_act
Pfam PF05860
Pfam clan CL0268
InterPro IPR008638
SCOP 1rwr
SUPERFAMILY 1rwr

In molecular biology, the haemagglutination activity domain is a conserved protein domain found near the N terminus of a number of large, repetitive bacterial proteins, including many proteins of over 2500 amino acids. A number of the members of this family have been designated adhesins, filamentous haemagglutinins, haem/haemopexin-binding protein, etc. Members generally have a signal sequence, then an intervening region, then the region described in this entry. Following this region, proteins typically have regions rich in repeats but may show no homology between the repeats of one member and the repeats of another. This domain is suggested to be a carbohydrate-dependent haemagglutination activity site.[1]

In Bordetella pertussis, the infectious agent in childhood whooping cough, filamentous haemagglutinin (FHA) is a surface-exposed and secreted protein that acts as a major virulence attachment factor, functioning as both a primary adhesin and an immunomodulator to bind the bacterial to cells of the respiratory epithelium.[2] The FHA molecule has a globular head that consists of two domains: a shaft and a flexible tail. Its sequence contains two regions of tandem 19-residue repeats, where the repeat motif consists of short beta-strands separated by beta-turns.[3]

References[edit]

  1. ^ Kajava AV, Cheng N, Cleaver R, Kessel M, Simon MN, Willery E, Jacob-Dubuisson F, Locht C, Steven AC (October 2001). "Beta-helix model for the filamentous haemagglutinin adhesin of Bordetella pertussis and related bacterial secretory proteins". Mol. Microbiol. 42 (2): 279–92. doi:10.1046/j.1365-2958.2001.02598.x. PMID 11703654. 
  2. ^ Inatsuka CS, Julio SM, Cotter PA (December 2005). "Bordetella filamentous hemagglutinin plays a critical role in immunomodulation, suggesting a mechanism for host specificity". Proc. Natl. Acad. Sci. U.S.A. 102 (51): 18578–83. doi:10.1073/pnas.0507910102. PMC 1317942Freely accessible. PMID 16339899. 
  3. ^ Makhov AM, Hannah JH, Brennan MJ, Trus BL, Kocsis E, Conway JF, Wingfield PT, Simon MN, Steven AC (August 1994). "Filamentous hemagglutinin of Bordetella pertussis. A bacterial adhesin formed as a 50-nm monomeric rigid rod based on a 19-residue repeat motif rich in beta strands and turns". J. Mol. Biol. 241 (1): 110–24. doi:10.1006/jmbi.1994.1478. PMID 7519681. 

This article incorporates text from the public domain Pfam and InterPro IPR008638