Immunoscreening

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Immunoscreening is a method of biotechnology to detect a polypeptide produced from a cloned gene.

Clones are screened for the presence of the gene product (a protein). This strategy requires first that a gene library is implemented in an expression vector, and that antiserum to the protein is available. Radioactivity or an enzyme is coupled generally with the secondary antibody. The radioactivity/enzyme linked secondary antibody can be purchased commercially and can detect different antigens. In commercial diagnostics labs, labelled primary antibodies are also used.[1]

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References[edit]

  1. ^ Karam, James (November 26, 1990). Methods in Nucleic Acids Research. CRC Press. p. 309. ISBN 0849353114.