Enzyme
|
PDB code
|
Source
|
Recognition sequence
|
Cut
|
Isoschizomers
|
Bsp6I
|
|
Bacillus sp. RFL6
|
5' GCNGC 3' CGNCG
|
5' ---GC NGC--- 3' 3' ---CGN CG--- 5'
|
|
Bsp13I
|
|
Bacillus sp. 13
|
5' TCCGGA 3' AGGCCT
|
5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5'
|
AccIII, BbvAIII, BlfI, BsiMI, BspMII, Bsu23I, Kpn2I, PinBII
|
Bsp19I
|
|
Bacillus sp. 19
|
5' CCATGG 3' GGTACC
|
5' ---C CATGG--- 3' 3' ---GGTAC C--- 5'
|
|
Bsp24I
|
|
Bacillus sp. 24
|
5' GACN6TGG 3' CTGN6ACC
|
5' ---GACN6TGGN6NNNNNN --- 3' 3' ---CTGN6ACCN6N NNNNN--- 5'
|
|
Bsp50I
|
|
Bacillus sp. RFL50
|
5' CGCG 3' GCGC
|
5' ---CG CG--- 3' 3' ---GC GC--- 5'
|
BceBI, Bsh1236I, BstFNI, BstUI, Bsu1532I, FalII, FnuDII, MvnI, ThaI
|
Bsp63I
|
|
Bacillus sphaericus 63
|
5' CTGCAG 3' GACGTC
|
5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5'
|
AjoI, Asp713I, BloHII, MhaAI, Pfl21I, PstI, SalPI, SflI, Sst12I, YenI
|
Bsp67I
|
|
Bacillus sphaericus 67
|
5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
AspMDI, Bsp143I, BspJI, BtkII, DpnII, LlaAI, NlaII, SsiBI
|
Bsp68I
|
|
Bacillus sp. RFL68
|
5' TCGCGA 3' AGCGCT
|
5' ---TCG CGA--- 3' 3' ---AGC GCT--- 5'
|
NruI
|
Bsp98I
|
|
Bacillus sp. RFL98
|
5' GGATCC 3' CCTAGG
|
5' ---G GATCC--- 3' 3' ---CCTAG G--- 5'
|
AccEBI, AliI, BamHI, BnaI, BstI, CelI, OkrAI, NspSAIV, Pfl8I, SurI
|
Bsp105I
|
|
Bacillus sphaericus 105
|
5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
Bfi57I, Bsp143I, BspJI, BtkII, DpnII, LlaAI, NlaII, SsiBI
|
Bsp106I
|
|
Bacillus sphaericus 106
|
5' ATCGAT 3' TAGCTA
|
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
|
BbvAII, Bci29I, BciBI, BcmI, BspOVII, BstNZ169I, Rme21I
|
Bsp119I
|
|
Bacillus sp. RFL119
|
5' TTCGAA 3' AAGCTT
|
5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5'
|
AcpI, Bim19I, Bpu14I, BspT104I, Csp45I, FspII, NspV, SspRFI
|
Bsp120I
|
|
Bacillus sp. RFL120
|
5' GGGCCC 3' CCCGGG
|
5' ---G GGCCC--- 3' 3' ---CCCGG G--- 5'
|
ApaI, PpeI, PspOMI
|
Bsp123I
|
|
Bacillus sp. 123
|
5' CGCG 3' GCGC
|
5' ---CG CG--- 3' 3' ---GC GC--- 5'
|
BceBI, Bsh1236I, BstFNI, BstUI, BtkI, Csp68KVI, FnuDII, MvnI, ThaI
|
Bsp143I
|
|
Bacillus sp. RFL143
|
5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
Bce243I, Bsp105I, BspFI, BstKTI, CpfI, HacI, NdeII, Sth368I, Sau3AI
|
Bsp143II
|
|
Bacillus sp. RFL143
|
5' RGCGCY 3' YCGCGR
|
5' ---RGCGC Y--- 3' 3' ---Y CGCGR--- 5'
|
AccB2I,
|
BfoI,
|
Bme142I, BstH2I,
|
HaeII, LpnI
|
|
Bsp211I
|
|
Bacillus sphaericus 211
|
5' GGCC 3' CCGG
|
5' ---GG CC--- 3' 3' ---CC GG--- 5'
|
|
Bsp423I
|
|
Bacillus sp. 423
|
5' GCAGC 3' CGTCG
|
5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5'
|
AlwXI, BbvI, BseKI, BseXI, Bst12I, Bst71I, BstV1I
|
Bsp519I
|
|
Bacillus sp. 519
|
5' GRGCYC 3' CYCGRG
|
5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5'
|
|
Bsp1286I
|
|
Bacillus sphaericus
|
5' GDGCHC 3' CHCGDG
|
5' ---GDGCH C--- 3' 3' ---C HCGDG--- 5'
|
AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, NspII, SduI
|
Bsp1407I
|
|
Bacillus stearothermophilus RFL1407
|
5' TGTACA 3' ACATGT
|
5' ---T GTACA--- 3' 3' ---ACATG T--- 5'
|
AauI,
|
BsmRI,
|
BsrGI, BstAUI,
|
Ssp4800I, SspBI
|
|
Bsp1720I
|
|
Bacillus sp. 1720
|
5' GCTNAGC 3' CGANTCG
|
5' ---GC TNAGC--- 3' 3' ---CGANT CG--- 5'
|
|
Bsp1894I
|
|
Bacillus sphaericus 1894
|
5' GGNCC 3' CCNGG
|
5' ---G GNCC--- 3' 3' ---CCNG G--- 5'
|
AspS9I, Bac36I, BavBII, BshKI, BspBII, CcuI, MaeK81II, Pde12I
|
Bsp2095I
|
|
Bacillus sphaericus 2095
|
5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
Bfi57I, Bsp143I, BspKT6I, BtkII, DpnII, LlaAI, NlaII, SsiBI
|
Bsp4009I
|
|
Bacillus sp. 4009
|
5' GGATCC 3' CCTAGG
|
5' ---G GATCC--- 3' 3' ---CCTAG G--- 5'
|
AccEBI, AliI, ApaCI, BamHI, BstI, CelI, OkrAI, Pfl8I, RspLKII, SurI
|
BspAI
|
|
Bacillus sphaericus JL4B
|
5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
Bfi57I, Bsp2095I, BspKT6I, BtkII, DpnII, LlaAI, NlaII, SsiBI
|
BspA2I
|
|
Bacillus sp. A2
|
5' CCTAGG 3' GGATCC
|
5' ---C CTAGG--- 3' 3' ---GGATC C--- 5'
|
AspA2I, AvrII, AvrBII, BlnI, XmaJI
|
Bsp153AI
|
|
Bacillus sp. 153A
|
5' CAGCTG 3' GTCGAC
|
5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5'
|
|
BspAAI
|
|
Bacillus sp. AA
|
5' CTCGAG 3' GAGCTC
|
5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5'
|
AbrI, BluI, BstVI, PanI, SauLPII, SciI, SlaI, StrI, TliI
|
BspAAII
|
|
Bacillus sp. AA
|
5' TCTAGA 3' AGATCT
|
5' ---T CTAGA--- 3' 3' ---AGATC T--- 5'
|
|
BspAAIII
|
|
Bacillus sp. AA
|
5' GGATCC 3' CCTAGG
|
5' ---G GATCC--- 3' 3' ---CCTAG G--- 5'
|
AccEBI, AliI, ApaCI, AsiI, BamHI, Bce751I, Pfl8I, RspLKII, SolI
|
BspANI
|
|
Bacillus sp. AN
|
5' GGCC 3' CCGG
|
5' ---GG CC--- 3' 3' ---CC GG--- 5'
|
|
BspBI
|
|
Bacillus sphaericus JL14
|
5' CTGCAG 3' GACGTC
|
5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5'
|
AjoI, ApiI, Asp713I, BloHII, CfrA4I, Pfl21I, PstI, SalPI, Sst12I, YenI
|
BspBII
|
|
Bacillus sphaericus JL14
|
5' GGNCC 3' CCNGG
|
5' ---G GNCC--- 3' 3' ---CCNG G--- 5'
|
AsuI, Bac36I, BavBII, BshKI, BspF4I, CcuI, MaeK81II, PspPI
|
BspBRI
|
|
Bacillus sp.
|
5' GGCC 3' CCGG
|
5' ---GG CC--- 3' 3' ---CC GG--- 5'
|
|
BspBS31I
|
|
Bacillus sp. BS31
|
5' GAAGAC 3' CTTCTG
|
5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5'
|
|
BspCI
|
|
Bacillus sp. C1
|
5' CGATCG 3' GCTAGC
|
5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5'
|
Afa16RI, Afa22MI, EagBI, ErhB9I, NblI, Psu161I, RshI, XorII
|
BspCNI
|
|
Bacillus sp. 1310
|
5' CTCAG 3' GAGTC
|
5' ---CTCAGN6NNN --- 3' 3' ---GAGTCN6N NN--- 5'
|
|
BspDI
|
|
Bacillus sp.
|
5' ATCGAT 3' TAGCTA
|
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
|
AagI, BanIII, BavCI, Bsa29I, BseCI, Bsu15I, BsuTUI, ClaI, ZhoI
|
BspD6I[7][8]
|
2P14
|
Bacillus sp. D6
|
5' GACTC 3' CTGAG
|
5' ---GACTCNNNN NN--- 3' 3' ---CTGAGNNNNNN --- 5'
|
|
BspEI
|
|
Bacillus sp.
|
5' TCCGGA 3' AGGCCT
|
5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5'
|
AccIII, BlfI, BsiMI, Bsp13I, BspMII, CauB3I, MroI, PinBII
|
BspFI
|
|
Bacillus sp.
|
5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
Bfi57I, Bsp2095I, BspKT6I, BtkII, DpnII, LlaAI, NlaII, SsiBI
|
BspF4I
|
|
Bacillus sp. F4
|
5' GGNCC 3' CCNGG
|
5' ---G GNCC--- 3' 3' ---CCNG G--- 5'
|
AsuI, Bac36I, BavBII, BsiZI, BspBII, CcuI, MaeK81II, PspPI
|
BspHI
|
|
Bacillus sp. H3
|
5' TCATGA 3' AGTACT
|
5' ---T CATGA--- 3' 3' ---AGTAC T--- 5'
|
|
BspIS4I
|
|
Bacillus sp. IS4
|
5' GAAGAC 3' CTTCTG
|
5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5'
|
|
BspJI
|
|
Bacillus sp.
|
5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
BfuCI, Bsp2095I, BspKT6I, BtkII, DpnII, LlaAI, NmeCI, SsiAI
|
BspJII
|
|
Bacillus sp.
|
5' ATCGAT 3' TAGCTA
|
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
|
Bsa29I, BseCI, Bsp106I, BspDI, BspOVII, BspXI, BspZEI
|
BspKI
|
|
Bacillus sp. K
|
5' GGCC 3' CCGG
|
5' ---GG CC--- 3' 3' ---CC GG--- 5'
|
|
BspKT5I
|
|
Bacillus sp.
|
5' CTGAAG 3' GACTTC
|
5' ---CTGAAGN12NNNN --- 3' 3' ---GACTTCN12NN NN--- 5'
|
|
BspKT6I
|
|
Bacillus sp. KT6
|
5' GATC 3' CTAG
|
5' ---GAT C--- 3' 3' ---C TAG--- 5'
|
BfuCI, Bsp2095I, Bst19II, BstKTI, DpnII, LlaAI, NmeCI, SsiAI
|
BspKT8I
|
|
Bacillus sp. KT8
|
5' AAGCTT 3' TTCGAA
|
5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5'
|
|
BspLI
|
|
Bacillus sp. RJ3-212
|
5' GGNNCC 3' CCNNGG
|
5' ---GGN NCC--- 3' 3' ---CCN NGG--- 5'
|
AspNI, BscBI,
|
BmiI,
|
NlaIV,
|
PspN4I
|
|
BspLAI
|
|
Bacillus sp. LA
|
5' GCGC 3' CGCG
|
5' ---GCG C--- 3' 3' ---C GCG--- 5'
|
AspLEI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNI
|
BspLAII
|
|
Bacillus sp. LA
|
5' TTCGAA 3' AAGCTT
|
5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5'
|
|
BspLAIII
|
|
Bacillus sp. LA
|
5' AAGCTT 3' TTCGAA
|
5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5'
|
|
BspLS2I
|
|
Bacillus sp. LS2
|
5' GDGCHC 3' CHCGDG
|
5' ---GDGCH C--- 3' 3' ---C HCGDG--- 5'
|
AocII, BmyI, BsoCI, BspLS2I, MhlI, NspII, SduI
|
BspLU4I
|
|
Bacillus sp. LU4
|
5' CYCGRG 3' GRGCYC
|
5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5'
|
Ama87I, AvaI, Bse15I, BsoBI, BstSI, NspIII, NspSAI, OfoI
|
BspLU11I
|
|
Bacillus sp. LU11
|
5' ACATGT 3' TGTACA
|
5' ---A CATGT--- 3' 3' ---TGTAC A--- 5'
|
|
BspLU11III
|
|
Bacillus sp. LU11
|
5' GGGAC 3' CCCTG
|
5' ---GGGACN8NN NNNN--- 3' 3' ---CCCTGN8NNNNNN --- 5'
|
|
BspMI
|
|
Bacillus sp. M
|
5' ACCTGC 3' TGGACG
|
5' ---ACCTGCNNNN NNNN--- 3' 3' ---TGGACGNNNNNNNN --- 5'
|
Acc36I, BfuAI, BveI
|
BspMII
|
|
Bacillus sp. M
|
5' TCCGGA 3' AGGCCT
|
5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5'
|
AccIII, BbvAIII, BlfI, BsiMI, BspEI, Bsu23I, CauB3I, MroI
|
BspM39I
|
|
Bacillus sp. M39
|
5' CAGCTG 3' GTCGAC
|
5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5'
|
|
BspM90I
|
|
Bacillus sp. M90
|
5' GTATAC 3' CATATG
|
5' ---GTA TAC--- 3' 3' ---CAT ATG--- 5'
|
|
BspMAI
|
|
Bacillus stearothermophilus MA
|
5' CTGCAG 3' GACGTC
|
5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5'
|
AjoI, Asp713I, BloHII, PaePI, Psp23I, PstI, SalPI, Sst12I, YenI
|
BspMKI
|
|
Bacillus sp. MKI
|
5' GTCGAC 3' CAGCTG
|
5' ---G TCGAC--- 3' 3' ---CAGCT G--- 5'
|
|
BspNI
|
|
Bacillus sp. N
|
5' CCWGG 3' GGWCC
|
5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
|
AglI, BseBI, Bse17I, Bst2I, CbrI, CthII, EcoRII, MvaI, SspAI, TaqXI
|
BspO4I
|
|
Bacillus sp. O-4
|
5' CAGCTG 3' GTCGAC
|
5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5'
|
|
BspOVI
|
|
Bacillus sp. OV
|
5' GACN5GTC 3' CTGN5CAG
|
5' ---GACNNN NNGTC--- 3' 3' ---CTGNN NNNCAG--- 5'
|
AhdI, AspEI,
|
BmeRI,
|
DriI,
|
Eam1105I, EclHKI, NruGI
|
|
BspOVII
|
|
Bacillus sp. OV
|
5' ATCGAT 3' TAGCTA
|
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
|
BanIII, BavCI, BciBI, BcmI, Bli86I, BliRI, BspJII, BstNZ169I, SpmI
|
BspPI
|
|
Bacillus sp. d 1-34
|
5' GGATC 3' CCTAG
|
5' ---GGATCNNNN N--- 3' 3' ---CCTAGNNNNN --- 5'
|
AclWI, AlwI, BinI,
|
BsrWI,
|
BstH9I,
|
Bst31TI, Bth617I,
|
EacI
|
|
BspRI
|
|
Bacillus sphaericus R
|
5' GGCC 3' CCGG
|
5' ---GG CC--- 3' 3' ---CC GG--- 5'
|
|
BspR7I
|
|
Bacillus sp. R7
|
5' CCTNAGG 3' GGANTCC
|
5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5'
|
AocI, AxyI, Bse21I, Bsu36I, Eco81I, MstII, SauI, SshAI
|
BspST5I
|
|
Bacillus sp. ST5
|
5' GCATC 3' CGTAG
|
5' ---GCATCN4N NNNN--- 3' 3' ---CGTAGN4NNNNN --- 5'
|
|
BspTI
|
|
Bacillus sp. RFL1265
|
5' CTTAAG 3' GAATTC
|
5' ---C TTAAG--- 3' 3' ---GAATT C--- 5'
|
AflII
|
BspT104I
|
|
Bacillus sp. T104
|
5' TTCGAA 3' AAGCTT
|
5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5'
|
AcpI, Bim19I, Bpu14I, BstBI, Csp45I, LspI, NspV, SspRFI
|
BspT107I
|
|
Bacillus sp. T107
|
5' GGYRCC 3' CCRYGG
|
5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5'
|
AccB1I, BanI, BbvBI, Eco64I, HgiCI, HgiHI, MspB4I, PfaAI
|
BspTNI
|
|
Bacillus sp. TN
|
5' GGTCTC 3' CCAGAG
|
5' ---GGTCTCN NNNN--- 3' 3' ---CCAGAGNNNNN --- 5'
|
|
BspTS514I
|
|
Bacillus sp. TS514
|
5' GAAGAC 3' CTTCTG
|
5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5'
|
|
BspWI
|
|
Bacillus sp. 3456
|
5' GCN7GC 3' CGN7CG
|
5' ---GCNNNNN NNGC--- 3' 3' ---CGNN NNNNNCG--- 5'
|
|
BspXI
|
|
Bacillus sphaericus X
|
5' ATCGAT 3' TAGCTA
|
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
|
BliAI, BliRI, BscI, Bst28I, Bsu15I, LcaI, PgaI, Ssp27144I, ZhoI
|
BspXII
|
|
Bacillus sphaericus X
|
5' TGATCA 3' ACTAGT
|
5' ---T GATCA--- 3' 3' ---ACTAG T--- 5'
|
AbaI, BclI, BsiQI,
|
BstT7I, FbaI, Ksp22I,
|
ParI
|
|
BspZEI
|
|
Bacillus sp. ZE
|
5' ATCGAT 3' TAGCTA
|
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
|
Bsa29I, BseCI, BspDI, BspXI, Bst28I, BstNZ169I, BsuTUI, LplI
|