MeRIPseq
MeRIPseq [1] stands for methylated RNA immunoprecipitation sequencing, which is a method for detection of post-transcriptional RNA modifications. It is also called m6A-seq.[2]
A variation of the MerIP-seq method was coined by Benjamin Delatte and colleagues in 2016. This variant, called hMerIP-seq (hydroxymethylcytosine RNA immunoprecipitation), uses an antibody that specifically recognizes 5-hydroxymethylcytosine, a modified RNA base affecting in vitro translation and brain development in Drosophila.[3]
References
- ^ Meyer, Kate D.; Saletore, Yogesh; Zumbo, Paul; Elemento, Olivier; Mason, Christopher E.; Jaffrey, Samie R. (31 May 2012). "Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3′ UTRs and near Stop Codons". Cell. 149 (7): 1635–1646. doi:10.1016/j.cell.2012.05.003. PMC 3383396. PMID 22608085.
- ^ Dominissini, Dan (28 April 2012). "Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq". Nature. 485 (7397): 201–206. doi:10.1038/nature11112. PMID 22575960.
{{cite journal}}: Unknown parameter|coauthors=ignored (|author=suggested) (help) - ^ Delatte, Benjamin (2016). "Transcriptome-wide distribution and function of RNA hydroxymethylcytosine". Science. 351 (6270): 282–285. doi:10.1126/science.aac5253. PMID 26816380.