OxyS RNA

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OxyS RNA
RF00035.jpg
Identifiers
Symbol OxyS
Rfam RF00035
Other data
RNA type Gene; sRNA
Domain(s) Bacteria
SO 0000384

OxyS RNA is a small non-coding RNA which is induced in response to oxidative stress in Escherichia coli. This RNA acts as a global regulator to activate or repress the expression of as many as 40 genes, by an antisense mechanism, including the fhlA-encoded transcriptional activator and the rpoS-encoded sigma(s) subunit of RNA polymerase.[1] OxyS is bound by the Hfq protein, that increases the OxyS RNA interaction with its target messages.[2] Binding to Hfq alters the conformation of OxyS.[3] The 109 nucleotide RNA is thought to be composed of three stem-loops.

Target genes of OxyS[edit]

A number of additional targets were recently predicted and verified using microarray analysis.[4] These are listed below:

  • yobF - confirmed by Northern analysis
  • yaiZ
  • rumA
  • wrbA - confirmed by Northern analysis
  • ybaY - confirmed by Northern analysis

References[edit]

  1. ^ Altuvia, S; Zhang A; Argaman L; Tiwari A; Storz G (1998). "The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding". EMBO J. 17 (20): 6069–6075. doi:10.1093/emboj/17.20.6069. PMC 1170933Freely accessible. PMID 9774350. 
  2. ^ Zhang, A; Wassarman KM; Ortega J; Steven AC; Storz G (2002). "The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs". Mol Cell. 9 (1): 11–22. doi:10.1016/S1097-2765(01)00437-3. PMID 11804582. 
  3. ^ Henderson, CA; Vincent, HA; Casamento, A; Stone, CM; Phillips, JO; Cary, PD; Sobott, F; Gowers, DM; Taylor, JE; Callaghan, AJ (August 2013). "Hfq binding changes the structure of Escherichia coli small noncoding RNAs OxyS and RprA, which are involved in the riboregulation of rpoS.". RNA (New York, N.Y.). 19 (8): 1089–104. doi:10.1261/rna.034595.112. PMC 3708529Freely accessible. PMID 23804244. 
  4. ^ Tjaden B, Goodwin SS, Opdyke JA, et al. (2006). "Target prediction for small, noncoding RNAs in bacteria". Nucleic Acids Res. 34 (9): 2791–802. doi:10.1093/nar/gkl356. PMC 1464411Freely accessible. PMID 16717284. 

External links[edit]