|RNA type||Gene; sRNA|
OxyS RNA is a small non-coding RNA which is induced in response to oxidative stress in Escherichia coli. This RNA acts as a global regulator to activate or repress the expression of as many as 40 genes, by an antisense mechanism, including the fhlA-encoded transcriptional activator and the rpoS-encoded sigma(s) subunit of RNA polymerase. OxyS is bound by the Hfq protein, that increases the OxyS RNA interaction with its target messages. Binding to Hfq alters the conformation of OxyS. The 109 nucleotide RNA is thought to be composed of three stem-loops.
Target genes of OxyS
A number of additional targets were recently predicted and verified using microarray analysis. These are listed below:
- yobF - confirmed by Northern analysis
- wrbA - confirmed by Northern analysis
- ybaY - confirmed by Northern analysis
- Altuvia, S; Zhang A; Argaman L; Tiwari A; Storz G (1998). "The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding". EMBO J. 17 (20): 6069–6075. doi:10.1093/emboj/17.20.6069. PMC . PMID 9774350.
- Zhang, A; Wassarman KM; Ortega J; Steven AC; Storz G (2002). "The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs". Mol Cell. 9 (1): 11–22. doi:10.1016/S1097-2765(01)00437-3. PMID 11804582.
- Henderson, CA; Vincent, HA; Casamento, A; Stone, CM; Phillips, JO; Cary, PD; Sobott, F; Gowers, DM; Taylor, JE; Callaghan, AJ (August 2013). "Hfq binding changes the structure of Escherichia coli small noncoding RNAs OxyS and RprA, which are involved in the riboregulation of rpoS.". RNA (New York, N.Y.). 19 (8): 1089–104. doi:10.1261/rna.034595.112. PMC . PMID 23804244.
- Tjaden B, Goodwin SS, Opdyke JA, et al. (2006). "Target prediction for small, noncoding RNAs in bacteria". Nucleic Acids Res. 34 (9): 2791–802. doi:10.1093/nar/gkl356. PMC . PMID 16717284.
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