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PFO may refer to:
- Patent foramen ovale - a blood-flow pathway between a human heart's atriums, normally naturally closed after birth
- Personal Freedom Outreach - an Evangelical organization opposing cults
- Pittsburgh Film Office
- IATA code for Paphos International Airport
- Pathfinder Roleplaying Game - online version
- Persistent Felony Offender - a defendant accused under a three-strikes law
| disambiguation page lists articles associated with the title PFO.
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