PYLIS downstream sequence

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Pyrrolysine insertion sequence 1
Predicted secondary structure of PYLIS 1 RNA.
Symbol PYLIS 1
Rfam RF01982
Other data
RNA type Cis-regulatory element
Domain(s) Archaea

In biology, the PYLIS downstream sequence (PYLIS: pyrrolysine insertion sequence) is a stem-loop structure that appears on some mRNA sequences. This structural motif was previously thought to cause the UAG (amber) stop codon to be translated to the amino acid pyrrolysine instead of ending the protein translation.[1] [2][3] However, it has been shown that PYLIS has no effect upon the efficiency of the UAG suppression,[4] hence even its name is, in fact, incorrect.

See also[edit]


  1. ^ Namy O, Rousset JP, Napthine S, Brierley I (2004). "Reprogrammed genetic decoding in cellular gene expression". Molecular Cell. 13 (2): 157–68. doi:10.1016/S1097-2765(04)00031-0. PMID 14759362. 
  2. ^ Théobald-Dietrich A, Giegé R, Rudinger-Thirion J (2005). "Evidence for the existence in mRNAs of a hairpin element responsible for ribosome dependent pyrrolysine insertion into proteins". Biochimie. 87 (9-10): 813–7. doi:10.1016/j.biochi.2005.03.006. PMID 16164991. 
  3. ^ Zhang Y, Baranov PV, Atkins JF, Gladyshev VN (May 2005). "Pyrrolysine and selenocysteine use dissimilar decoding strategies". J. Biol. Chem. 280 (21): 20740–51. doi:10.1074/jbc.M501458200. PMID 15788401. 
  4. ^ Namy, Olivier; Zhou, Yu; Gundllapalli, Sarath; Polycarpo, Carla R.; Denise, Alain; Rousset, Jean-Pierre; Söll, Dieter; Ambrogelly, Alexandre (November 2007). "Adding pyrrolysine to the Escherichia coli genetic code". FEBS Letters. 581 (27): 5282–8. doi:10.1016/j.febslet.2007.10.022. PMID 17967457. Retrieved 18 October 2015. 

Further reading[edit]

  • Longstaff DG, Blight SK, Zhang L, Green-Church KB, Krzycki JA (January 2007). "In vivo contextual requirements for UAG translation as pyrrolysine". Mol. Microbiol. 63 (1): 229–41. doi:10.1111/j.1365-2958.2006.05500.x. PMID 17140411. 
  • Theil Have, C; Zambach, S; Christiansen, H (April 4, 2013). "Effects of using coding potential, sequence conservation and mRNA structure conservation for predicting pyrrolysine containing genes". BMC Bioinformatics. 14 (1): 118. doi:10.1186/1471-2105-14-118. PMID 23557142. 

External links[edit]