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{{Short description|Shallow dish used to hold cell cultures}}
[[File:Antonio e Biagio e Cesare Arrigo Petri dish.jpg|alt=|thumb|A glass Petri dish with culture]]

A '''Petri dish''' (alternatively known as a '''Petri plate''' or '''cell-culture dish''') is a shallow transparent lidded dish that biologists use to hold [[growth medium]] in which [[Cell (biology)|cells]] can be [[Microbiological culture|cultured]],<ref name=dubey>R. C. Dubey (2014): ''A Textbook Of Biotechnology For Class-XI'', 4th edition, p. 469. {{isbn|978-8121924177}}</ref><ref>{{cite book |title=Mosby's Dental Dictionary |edition=2nd |year=2008 |publisher=Elsevier |url=http://medical-dictionary.thefreedictionary.com/petri+plate |access-date=2010-02-11}}</ref> originally, cells of [[bacteria]], [[fungi]] and small [[moss]]es.<ref>{{cite journal|author=Ralf Reski |title=Development, genetics and molecular biology of mosses|journal=Botanica Acta|volume=111|year=1998|pages=1–15|url=https://www.plant-biotech.net/paper/Reski_1998_BotActa-111_1_scan.pdf|doi=10.1111/j.1438-8677.1998.tb00670.x|author-link=Ralf Reski|access-date=2015-07-19|archive-url=https://web.archive.org/web/20150924074016/http://www.plant-biotech.net/paper/Reski_1998_BotActa-111_1_scan.pdf|archive-date=2015-09-24|url-status=dead}}</ref> The container is named after its inventor, German [[bacteriologist]] [[Julius Richard Petri]].<ref>[http://education.yahoo.com/reference/dictionary/entry/Petri+dish Petri dish] {{Webarchive|url=https://web.archive.org/web/20131022180229/http://education.yahoo.com/reference/dictionary/entry/Petri+dish |date=2013-10-22 }} in the [[American Heritage Dictionary]].</ref><ref>{{cite journal |last1=Petri |first1=R.J. |title=Eine kleine Modification des Koch'schen Plattenverfahrens |journal=Centralblatt für Bakteriologie und Parasitenkunde |date=1887 |volume=1 |pages=279–80 |url=https://www.biodiversitylibrary.org/item/210666#page/313/mode/1up |trans-title=A small modification of Koch's plate method |language=de}}</ref><ref name=Petri>{{cite journal |last1=Petri |first1=R.J. |title=Eine kleine Modification des Koch'schen Plattenverfahrens |journal=Centralblatt für Bakteriologie und Parasitenkunde (English Translation, Braus, 2020) |date=1887 |volume=1 |pages=279–80 |url=https://archive.org/details/1887-petri-eine-kleine-modification-des-koch-schen-plattenverfahrens-2020-braus- |trans-title=A small modification of Koch's plate method |language=de}}</ref> It is the most common type of [[culture plate]]. The Petri dish is one of the most common items in biology laboratories and has entered [[popular culture]]. The term is sometimes written in lower case, especially in non-technical literature.<ref name="sing2018" /><ref name="slone2018" />

What was later called Petri dish was originally developed by German physician [[Robert Koch]] in his private laboratory in 1881, as a precursor method. Petri, as assistant to Koch, at Berlin University made the final modifications in 1887 as used today.

[[Penicillin]], the first antibiotic, was discovered in 1929 when [[Alexander Fleming]] noticed that penicillium [[Mold (fungus)|mold]] contaminating a [[bacteria]]l culture in a Petri dish had killed the bacteria around it.

== History ==

The Petri dish was developed by German physician [[Julius Richard Petri]] (after whom the name is given) while working as an assistant to [[Robert Koch]] at [[Berlin University]]. Petri did not invent the culture dish himself; rather, it was a modified version of Koch's invention<ref name=":7">{{Cite journal|last=Hufford|first=David C.|date=1988-03-01|title=A Minor Modification by R. J. Petri|url=https://academic.oup.com/labmed/article-lookup/doi/10.1093/labmed/19.3.169|journal=Laboratory Medicine|language=en|volume=19|issue=3|pages=169–170|doi=10.1093/labmed/19.3.169|issn=0007-5027}}</ref> which used an [[agar]] medium that was developed by Walther Hesse.<ref name="kass">{{cite book |last1=Kassinger |first1=Ruth |title=Slime How Algae Created Us, Plague Us, and Just Might Save Us |date=2019 |publisher=Houghton Mifflin Harcourt |location=Boston, New York |page=124}}</ref> Koch had published a precursor dish in a booklet in 1881 titled "{{Lang|de|Zur Untersuchung von Pathogenen Organismen}}" (''Methods for the Study of Pathogenic Organisms''),<ref>{{Cite book|last=Koch|first=Robert|url=https://edoc.rki.de/handle/176904/5146|title=Zur Untersuchung von Pathogenen Organismen| publisher=Robert Koch-Institut|year=2010|location=Berlin|doi=10.25646/5071|orig-year=1881}}</ref> which has been known as the "Bible of Bacteriology".<ref>{{Citation|last1=Booss|first1=John|title=A history of viral infections of the central nervous system|date=2014|url=https://linkinghub.elsevier.com/retrieve/pii/B9780444534880000018|series=Handbook of Clinical Neurology|volume=123|pages=3–44|publisher=Elsevier|language=en|doi=10.1016/b978-0-444-53488-0.00001-8|isbn=978-0-444-53488-0|pmid=25015479|access-date=2021-04-15|last2=Tselis|first2=Alex C.}}</ref><ref>{{Cite journal|last=Hurt|first=Leslie|date=2003|title=Dr. Robert Koch:: a founding father of biology|url= https://linkinghub.elsevier.com/retrieve/pii/S1068607X02001671|journal=Primary Care Update for OB/GYNS|language=en|volume=10|issue=2|pages=73–74|doi=10.1016/S1068-607X(02)00167-1}}</ref> He described a new bacterial culture method that used a glass slide with agar and a container (basically a Petri dish, a circular glass dish of 20 × 5 cm with matching lid) which he called ''{{Lang|de|feuchte Kammer}}'' ("moist chamber"). A bacterial culture was spread on the glass slide, then placed in the moist chamber with a small wet paper. Bacterial growth was easily visible.<ref name=":8">{{Cite journal|last=Shama|first=Gilbert|date=2019|title=The "Petri" Dish: A Case of Simultaneous Invention in Bacteriology|url= https://pubmed.ncbi.nlm.nih.gov/31030894|journal=Endeavour|volume=43|issue=1–2|pages=11–16|doi=10.1016/j.endeavour.2019.04.001|pmid=31030894|s2cid=139105012}}</ref>

Koch publicly demonstrated his plating method at the Seventh [[International Medical Congress]] in London in August 1881. There, [[Louis Pasteur]] exclaimed, "{{Lang|fr|C'est un grand progrès, Monsieur!}}" ("What a great progress, Sir!")<ref name=":2">{{Cite journal|last=Sakula|first=A.|date=1982|title=Robert Koch: centenary of the discovery of the tubercle bacillus, 1882|journal=Thorax|volume=37|issue=4|pages=246–251|doi= 10.1136/thx.37.4.246|pmc=459292|pmid=6180494}}</ref> It was using this method that Koch discovered important pathogens of [[tuberculosis]] (''[[Mycobacterium tuberculosis]]''), [[anthrax]] (''[[Bacillus anthracis]]''), and cholera (''[[Vibrio cholerae]]''). For his research on tuberculosis, he was awarded the [[Nobel Prize in Physiology or Medicine]] in 1905.<ref name="Robert Koch A Life">{{cite book |last1=Brock |first1=Thomas D. |title=Robert Koch: A Life in Medicine and Bacteriology |date=1999 |publisher=American Society of Microbiology |location=Washington, D.C. |doi=10.1128/9781555818272 |isbn=978-1-55581-143-3 |url= https://www.asmscience.org/content/book/10.1128/9781555818272 |language=en}}</ref> His students also made important discoveries. [[Friedrich Loeffler]] discovered the bacteria of [[glanders]] (''[[Burkholderia mallei]]'') in 1882 and [[diphtheria]] (''[[Corynebacterium diphtheriae]]'') in 1884; and [[Georg Theodor August Gaffky]], the bacterium of [[typhoid]] (''[[Salmonella enterica]]'') in 1884.<ref name=":6">{{Cite journal|last=Weiss|first=Robin A.|date= 2005|title=Robert Koch: the grandfather of cloning?|journal=Cell|volume=123|issue=4|pages=539–542|doi=10.1016/j.cell.2005.11.001|pmid=16286000|doi-access=free}}</ref>

Petri made changes in how the circular dish was used. It is often asserted that Petri developed a new culture plate,<ref name=":0">{{Cite journal|last1=Blevins|first1=Steve M.|last2=Bronze|first2=Michael S.|date=2010|title=Robert Koch and the 'golden age' of bacteriology|journal=International Journal of Infectious Diseases|volume=14|issue=9|pages=e744–751|doi=10.1016/j.ijid.2009.12.003|pmid=20413340|doi-access= free}}</ref><ref>{{Cite journal|last=Zhang|first=Shuguang|date=2004|title=Beyond the Petri dish|url=https://pubmed.ncbi.nlm.nih.gov/14755282|journal=Nature Biotechnology|volume=22|issue=2|pages=151–152|doi=10.1038/nbt0204-151|pmid=14755282|s2cid=36391864}}</ref><ref>{{Cite journal|last1=Grzybowski|first1=Andrzej|last2=Pietrzak|first2=Krzysztof|date=2014|title=Robert Koch (1843-1910) and dermatology on his 171st birthday|url=https://pubmed.ncbi.nlm.nih.gov/24887990|journal=Clinics in Dermatology|volume=32|issue=3|pages=448–450|doi=10.1016/j.clindermatol.2013.10.005|pmid=24887990}}</ref> but this is incorrect. Instead of using a separate glass slide or plate on which culture media were placed, Petri directly placed media into the glass dish, eliminating unnecessary steps such as transferring the culture media, using the wet paper, and reducing the chance of contamination.<ref name=":7" /> He published the improved method in 1887 as "''{{Lang|de|Eine kleine Modification des Koch’schen Plattenverfahrens}}''" ("A minor modification of the plating technique of Koch").<ref name=Petri/> Although it could have been named "Koch dish",<ref name=":8" /> the final method was given an eponymous name Petri dish.<ref>{{Cite journal|last=Mahajan|first=Monika|date=2021|title=Etymologia: Petri Dish|journal=Emerging Infectious Diseases|volume=27|issue=1|pages=261|doi=10.3201/eid2701.ET2701|issn=1080-6040|pmc=7774570}}</ref>

== Features and variants ==
{{Cite check section|date=March 2020|reason=every citation is a page to purchase one of the dishes. Needs publications stating the relevant statements instead.}}

Petri dishes are usually [[cylinder (geometry)|cylindrical]], mostly with diameters ranging from {{convert|30|to|200|mm}},<ref name=fisch4909050/><ref>(2019): "[https://www.auxilab.es/en/laboratory-equipment/petri-dish-200-mm-borosilicate/ Product BRB011: Petri Dish 200 mm, borosilicate]". Rogo-Sampaic online catalog. Accessed on 2019-10-25.</ref> and a height to diameter ratio ranging from 1:10 to 1:4.<ref>(2019): "[https://www.fishersci.com/shop/products/corning-gosselin-100-x-25mm-bio-agricultural-petri-dishes-2/p-4494233 Product BTX9302 Corning 100 x 25mm bio-agricultural Petri dishes]". Fischer Scientific online catalog. Accessed on 2019-10-25.</ref> Squarish versions are also available.<ref name=thomas1219C98>(2019): "[https://www.thomassci.com/E-K/Cell-Culture/Dishes/_/Square-Petri-Dish-w/-Grid1?q=Vented%20Petri%20Dish Item 1219C98: Square Petri dish w/ grid]". Thomas Scientific online catalog. Accessed on 2019-10-25.</ref><ref name=fisch11708573>(2019): "[https://www.fishersci.co.uk/shop/products/gosselin-square-petri-dish/11708573 Product 11708573: Gosselin Square Petri Dish]". Fischer Scientific online catalog. Accessed on 2019-10-25.</ref>

Petri dishes were traditionally reusable and made of [[glass]]; often of heat-resistant [[borosilicate glass]] for proper [[Sterilization (microbiology)|sterilization]] at 120–160 [[celsius|°C]].<ref name=fisch4909050>(2019): "[https://www.fishersci.com/shop/products/pyrex-reusable-petri-dishes-complete-6/p-4909050 Product 4909050: PYREX reusable Petri dishes: complete]". Fischer Scientific online catalog. Accessed on 2019-10-25.</ref>

Since the 1960s, [[plastic]] dishes, usually disposable, are also common.<ref name=fischBP94S01>(2019): "[https://www.fishersci.com/shop/products/corning-gosselin-100-x-15mm-polystyrene-petri-dishes/p-5162666 Product BP94S01: Corning 100 x 15mm Polystyrene Petri Dishes]". Fischer Scientific online catalog. Accessed on 2019-10-25.</ref>

The dishes are often covered with a shallow transparent lid, resembling a slightly wider version of the dish itself.  The lids of glass dishes are usually loose-fitting.<ref name=fisch4909050/>  Plastic dishes may have close-fitting covers that delay the drying of the contents.<ref name=fisch09-720-500>(2019): "[https://www.fishersci.com/shop/products/fisherbrand-disposable-petri-dishes-pad-dishes-8/p-3219248 Item 09-720-500: Fisherbrand disposable Petri dishes]". Fischer Scientific online catalog. Accessed on 2019-10-25.</ref>   Alternatively, some glass or plastic versions may have small holes around the rim, or ribs on the underside of the cover, to allow for air flow over the culture and prevent [[Water vapor#Condensation|water condensation]].<ref name=fischSB93102>(2019): "[https://www.fishersci.com/shop/products/gosselin-100x15mm-petri-dish-three-vents-optimized-automation-2/p-8015709 Item SB93102: Corning 100x15mm Petri dish with three vents]". Fischer Scientific online catalog. Accessed on 2019-10-25.</ref>

Some Petri dishes, especially plastic ones, usually feature rings and/or slots on their lids and bases so that they are less prone to sliding off one another when stacked or sticking to a smooth surface by suction.<ref name=fisch09-720-500/>

Small dishes may have a protruding base that can be secured on a [[microscope]] stage for direct examination.<ref name=fischPD1504700>(2019): "[https://www.fishersci.com/shop/products/emd-millipore-petrislide-containers/PD1504700 Product PD1504700 MilliporeSigma PetriSlide for contamination analysis]". Fischer Scientific online catalog. Accessed on 2019-10-25.</ref>

Some versions may have grids printed on the bottom to help in measuring the density of cultures.<ref name=hecht41044  >(2019): "[https://www.assistent.eu/en/product/petri-dishes-made-of-glass-with-grid-and-cover/ Item 41044: Petri dishes made of glass with grid and cover]". Assistent (Karl Hecht) online catalog. Accessed on 2019-10-25</ref><ref name=thomas1219C98/><ref name=fisch11708573/>

A [[microplate]] is a single container with an array of flat-bottomed cavities, each being essentially a small Petri dish.  It makes it possible to inoculate and grow dozens or hundreds of independent cultures of dozens of samples at the same time.  Besides being much cheaper and convenient than separate dishes, the microplate is also more amenable to automated handling and inspection.

Some plates are separated into different mediums known as biplates, triplates, and quadplates.

== Uses ==
[[File:Agar plate with colonies.jpg|thumb|A Petri dish with [[bacteria]]l colonies on an [[agar]]-based [[growth medium]]]]
[[File:Physcomitrella_growing_on_agar_plates.jpg|thumb|Axenic [[cell culture]] of the plant ''[[Physcomitrella patens]]'' on an [[agar]]plate in a Petri dish]]

Petri dishes are widely used in biology to [[microbiological culture|cultivate]] microorganisms such as [[bacterium|bacteria]], [[yeast]]s, and [[Mold (fungus)|mold]]s.  It is most suited for organisms that thrive on a solid or semisolid surface. The [[growth medium|culture medium]] is often an [[agar plate]], a layer a few mm thick of [[agar]] or [[agarose]] [[hydrogel|gel]] containing whatever nutrients the organism requires (such as [[blood]], [[salt (chemistry)|salts]], [[carbohydrate]]s, [[amino acid]]s) and other desired ingredients (such as [[staining|dyes]], [[chemical indicator|indicators]], and [[medicinal drug]]s). The agar and other ingredients are dissolved in warm water and poured into the dish and left to cool down.  Once the medium solidifies, a sample of the organism is [[Inoculation|inoculated]] ("plated"). The dishes are then left undisturbed for hours or days while the organism grows, possibly in an [[Incubator (culture)|incubator]].  They are usually covered, or placed upside-down, to lessen the risk of contamination from airborne [[spore]]s. [[Virus]] or [[phage]] cultures require that a population of bacteria be grown in the dish first, which then becomes the culture medium for the viral inoculum.

While Petri dishes are widespread in microbiological research, smaller dishes tend to be used for large-scale studies in which growing cells in Petri dishes can be relatively expensive and labor-intensive.<ref>{{cite journal|author=Gilbert, P.M. |title=Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture|doi=10.1126/science.1191035 |journal=Science|volume= 329 |issue=5995|year=2010|pages=1078–81 |pmid=20647425 |pmc=2929271|bibcode=2010Sci...329.1078G}}</ref><ref>{{cite journal|author=Chowdhury, F.|title= Soft substrates promote homogeneous self-renewal of embryonic stem cells via downregulating cell-matrix tractions|journal=PLOS ONE |volume=5|issue= 12|pages= e15655 |year=2010|doi= 10.1371/journal.pone.0015655|pmid=21179449|pmc=3001487
|bibcode= 2010PLoSO...515655C|doi-access= free}}</ref>

Petri dishes can be used to visualize the location of contamination on surfaces, such as kitchen counters and utensils,<ref>{{cite journal|doi=10.1078/S1438-4639(04)70035-8|pmid=11279821|title=Comparison of two sampling methods for the detection of Gram-positive and Gram-negative bacteria in the environment: Moistened swabs versus Rodac plates|journal=International Journal of Hygiene and Environmental Health|volume=203|issue=3|pages=245–48|year=2001|last1=Lemmen|first1=Sebastian W.|last2=Häfner|first2=Helga|last3=Zolldann|first3=Dirk|last4=Amedick|first4=Günter|last5=Lutticken|first5=Rüdolf}}</ref> clothing, food preparation equipment, or animal and human skin.<ref>Kasia Galazka (2015): "[https://www.buzzfeednews.com/article/kasiagalazka/petri-handprint Here's A Gorgeous Petri Dish Handprint Of An 8-Year-Old After He Played Outside]". BuzzFeed.News online article, June 9, 2015.  Accessed on 2019-10-25.</ref><ref>Sonja Bäumel (2009): "[http://www.sonjabaeumel.at/work/bacteria/oversized-petri-dish Oversized petri dish]". Culture of microorganisms from the artist's skin pressed onto a body-size culture plate, photographed over the span of 44 days. Part of her ''(In)visible membrane'' project. Wageningen, Germany. Accessed on 2019-10-25.</ref> For this application, the Petri dishes may be filled so that the culture medium protrudes slightly above the edges of the dish to make it easier to take samples on hard objects.  Shallow Petri dishes prepared in this way are called [[RODAC plate|Replicate Organism Detection And Counting (RODAC) plates]] and are available commercially.<ref name=sutt2007>Scott Sutton (2007): "Microbial Surface Monitoring", p. 78. Chapter 5 of Anne Marie Dixon (ed.), ''Environmental Monitoring for Cleanrooms and Controlled Environments''. {{isbn|978-1420014853}}</ref><ref name=dane2016>Géraldine Daneau, Elie Nduwamahoro, Kristina Fissette, Patrick Rüdelsheim, Dick van Soolingen, Bouke C. de Jong, Leen Rigouts (2016): "Use of RODAC plates to measure containment of Mycobacterium tuberculosis in a Class IIB biosafety cabinet during routine operations." ''International Journal of Mycobacteriology'', volume 5, issue 2, pp. 148–54. {{doi|10.1016/j.ijmyco.2016.01.003}}</ref>

Petri dishes are also used for [[cell culture|cell cultivation]] of isolated cells from [[Eukaryote|eukaryotic]] organisms, such as in [[immunodiffusion]] studies, on solid agar or in a liquid medium.

Petri dishes may be used to observe the early stages of plant [[germination]], and to grow plants asexually from isolated cells.

Petri dishes may be convenient enclosures to study the behavior of insects and other small animals.

Due to their large open surface, Petri dishes are effective containers to evaporate [[solvent]]s and dry out [[precipitate]]s, either at room temperature or in [[oven]]s and [[desiccator]]s.

Petri dishes also make convenient temporary storage for samples, especially liquid, granular, or powdered ones, and small objects such as insects or seeds.  Their transparency and flat profile allows the contents to be inspected with the naked eye, [[magnifying glass]], or low-power microscope without removing the lid.

== In popular culture ==

The Petri dish is one of a small number of laboratory equipment items whose name entered popular culture.  It is often used metaphorically, e.g. for a contained community that is being studied as if they were microorganisms in a biology experiment, or an environment where original ideas and enterprises may flourish.<ref name=sing2018>Gary Singer (2018): "[https://books.google.com/books?id=PmiCDwAAQBAJ&pg=PT40 Sonder, in the City]". Quote: ''As a native New Yorker, I tend to think of this city as a giant petri dish, in which some of the greatest breakthroughs, inventions, and audacious ideas have been nurtured to fruition.'' In Angela Dews (ed.)  ''Still, in the City: Creating Peace of Mind in the Midst of Urban Chaos'', p. 40. {{isbn|978-1510732346}}</ref><ref name=slone2018>Isabel Slone (2018): "[https://fashionmagazine.com/fashion/mall-goth/ What Does the Mall Goth Nostalgia Trend Really Mean?]". Quote: ''"mall goth" was a style of dress that combined the hallmarks of punk, goth and metal subcultures and thrived like bacteria in the petri dish of the early 2000s.'' Online article in the Fashion Magazine website, May 22, 2018. Accessed on 2019-10-25.</ref><ref name="merriam-webster.com/dictionary/petridish">{{Cite web | url=https://www.merriam-webster.com/dictionary/petri%20dish |title = Definition of petri dish}}</ref>

[[Unicode]] has a Petri dish [[emoji]], "<span style="font-size:larger;">🧫</span>", which has the [[code point]] U+1F9EB ([[HTML entity]] "&amp;#129515;" or "&amp;#x1F9EB;", [[UTF-8]] "0xF0 0x9F 0xA7 0xAB").<ref name="unicode">(2019): "[http://unicode.org/emoji/charts/emoji-list.html Emoji List, v12.1]". Webpage at the Unicode Consortium website. Accessed on 2019-10-25.</ref>

== See also ==

* {{anl|Microbial art}}
* {{anl|Cell spreader}}
* {{anl|Inoculation loop}}
* {{anl|Roux culture bottle}}

== References ==

{{refs}}

== External links ==

* {{commonscat-inline|Petri dishes}}

{{Laboratory equipment}}

[[Category:Laboratory glassware]]
[[Category:Microbiology equipment]]
[[Category:German inventions]]
[[Category:1887 in science]]
[[Category:1887 in Germany]]