Microbial art, agar art, or germ art is artwork created by culturing microorganisms in certain patterns. The microbes used can be bacteria, yeast fungi, or less commonly, protists. The microbes can be chosen for their natural colours, or can be engineered to express fluorescent proteins and viewed under ultraviolet light to make them fluoresce in colour.
Agar plates are used as a canvas, while pigmented or fluorescent bacteria and yeasts represent the paint. In order to preserve a piece of microbial art after a sufficient incubation, the microbe culture is sealed with epoxy.
Microbe species can be chosen for their natural colours to form a palette for the artwork. Suitable species of bacteria (with their colours) include Bacillus subtilis (cream to brown), Chromobacterium violaceum (violet), Escherichia coli (colourless), Micrococcus luteus (yellow), Micrococcus roseus (pink), Proteus mirabilis, Pseudomonas aeruginosa (brown), Pseudomonas fluorescens (naturally blue-green fluorescent with pyoverdine), Serratia marcescens (pink or orange), Staphylococcus aureus (yellow), and Vibrio fischeri (bioluminescent).
Yeast species – which are fungi – used include Saccharomyces cerevisiae (yellow-white) Aspergillus flavus (yellow-green spores), Aspergillus ochraceus (yellow), Aureobasidium pullulans (black), Candida albicans (whitish buff), Candida sake, Candida sp. (whitish), Cladosporium herbarum (brown to black), Cladosporium resinae, Epicoccum nigrum (yellow, orange, red, brown, and black), Fusarium sp., Rhodotorula sp., and Scopulariopsis brevicaulis.[a]
A technique called "bacteriography" involves selectively killing certain areas of a bacterial culture with radiation, in order to produce artistic patterns. After incubation, the culture is sealed with acrylic.
The biochemist Roger Tsien won the 2008 Nobel prize for chemistry for his contributions to knowledge of green fluorescent protein (GFP). A researcher in his laboratory, Nathan Shaner, made a 2006 microbial artwork using GFP of a San Diego beach scene.
Agar Art Competition
The American Society for Microbiology hosts an annual contest for microbial art: Agar Art. The contest was organized after a picture from a Christmas tree, made by Rositsa Tashkova, went viral in 2014. The 2015 edition covered 85 submissions, of which microbial art created by Mehmet Berkmen and Maria Peñil called Neurons won first place. They have been working together since 2011 on making bacterial art. The artwork used yellow Nesterenkonia and orange Deinococcus and Sphingomonas.
- The colour shown by the micro-organisms also depends on the medium used.
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