Pistol ribozyme
The pistol ribozyme is an RNA structure that catalyzes its own cleavage at a specific site. In other words, it is a self-cleaving ribozyme. The pistol ribozyme was discovered by a bioinformatics strategy [1] as an RNA Associated with Genes Associated with Twister and Hammerhead ribozymes, or RAGATH.
Subsequent biochemical analysis supports the conclusion of a ribozyme function, and determined further characteristics of the chemical reaction catalyzed by the ribozyme.[2] This understanding was further advanced by an atomic-resolution structured of a pistol ribozyme[3]
References
- ^ Weinberg Z, Kim PB, Chen TH, Li S, Harris KA, Lünse CE, Breaker RR (2015). "New classes of self-cleaving ribozymes revealed by comparative genomics analysis". Nat. Chem. Biol. 11 (8): 606–10. doi:10.1038/nchembio.1846. PMC 4509812. PMID 26167874.
- ^ Harris KA, Lünse CE, Li S, Brewer KI, Breaker RR (2015). "Biochemical analysis of pistol self-cleaving ribozymes". RNA. 21 (11): 1852–8. doi:10.1261/rna.052514.115. PMC 4604425. PMID 26385507.
- ^ Ren A, Vušurović N, Gebetsberger J, Gao P, Juen M, Kreutz C, Micura R, Patel DJ (2016). "Pistol ribozyme adopts a pseudoknot fold facilitating site-specific in-line cleavage". Nat. Chem. Biol. 12 (9): 702–8. doi:10.1038/nchembio.2125. PMID 27398999.