Scanning acoustic microscope
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A scanning acoustic microscope (SAM) is a device which uses focused sound to investigate, measure, or image an object (a process called scanning acoustic tomography). It is commonly used in failure analysis and non-destructive evaluation. It also has applications in biological and medical research. The semiconductor industry has found the SAM useful in detecting voids, cracks, and delaminations within microelectronic packages.
The first scanning acoustic microscope was developed in 1974 by R. A. Lemons and C. F. Quate at the Microwave Laboratory of Stanford University. Since then, many improvements to such systems have been made to enhance resolution and accuracy.
Principles of operation
Scanning acoustic microscopy works by directing focused sound from a transducer at a small point on a target object. Sound hitting the object is either scattered, absorbed, reflected (scattered at 180°) or transmitted (scattered at 0°). It is possible to detect the scattered pulses travelling in a particular direction. A detected pulse informs of the presence of a boundary or object. The `time of flight' of the pulse is defined as the time taken for it to be emitted by an acoustic source, scattered by an object and received by the detector, which is usually coincident with the source. The time of flight can be used to determine the distance of the inhomogeneity from the source given knowledge of the speed through the medium.
Based on the measurement, a value is assigned to the location investigated. The transducer (or object) is moved slightly and then insonified again. This process is repeated in a systematic pattern until the entire region of interest has been investigated. Often the values for each point are assembled into an image of the object. The contrast seen in the image is based either on the object's geometry or material composition. The resolution of the image is limited either by the physical scanning resolution or the width of the sound beam (which in turn is determined by the frequency of the sound).
SAM is used for counterfeit detection, product reliability testing, process validation, vendor qualification, quality control, failure analysis, research, and development. Detecting discontinuities in silicon is just one of the ways scanning acoustic microscopy is being used for testing in the semiconductor market.
Medicine and biology
SAM can provide data on the elasticity of cells and tissues, which can give useful information on the physical forces holding structures in a particular shape and the mechanics of structures such as the cytoskeleton. These studies are particularly valuable in investigating processes such as cell motility.
Some work has also been performed to assess penetration depth of particles injected into skin using needle-free injection 
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