Second line or variants thereof can refer to:
- Line (ice hockey), a second line is a hockey line generally composed of second-tier offensive players
- Second line (parades), a tradition in brass band parades in New Orleans, Louisiana, as well as an associated traditional dance style
- Second Line (shipping line), a shipping line also known as Robert Kermits Red Star Line or New Line
- Second-line therapy: Treatment that is given when initial treatment (first-line therapy) doesn’t work, or stops working.
- Line 2 - number of line in railway systems in some cities.
- National Cancer Institute > Dictionary of Cancer Terms > second-line therapy Retrieved July 2010
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