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Serious Business may refer to
- Serious Business (album), by Johnny Winter, released in 1985
- "Serious Business", a song from the album Uh-huh by John Cougar Mellencamp and the B-side of "Pink Houses"
- Serious Business, one of the earliest game developers on Facebook, acquired by Zynga in 2010
- Serious Business Records, a record label founded by Travis Harrison and Andy Ross
- SIRIUS Business, a commercial background music service of Applied Media Technologies Corporation