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The Southern Highlands could refer to:
- Southern Highlands, New South Wales, Australia, a small geographical and wine region located between Sydney and Canberra.
- Southern Highlands Province, Papua New Guinea
- Southern Highlands, Nevada, an affluent community in southwest Las Vegas
- Southern Highlands, Appalachian Mountains, southeast United States
- Southern Highlands, Tanzania, Africa, a region of rich biodiversity at the southern tip of the East African Rift
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