Talk:Reporter gene
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GFP Fluorescence Edit
[edit]I made an edit for scientific accuracy because green fluorescent protein (GFP) glows green under blue light as well as under ultraviolet light.
This is supported by the article that is cited which says "under illumination with the 360–400 nm spectrum of light that excites GFP fluorescence"[1] which corresponds to ultraviolet light.[2]
I don't believe that my edit would constitute a unit conversion—or at least, not one that is valid under WP:SYNTH—hence why I provided a citation. Overall, the citations for that line could be tidied up, but I think the best fix would be a single source that encompasses both the primary source and covers the range of light.
Kilvin the Futz-y Enterovirus (talk) 20:43, 18 March 2024 (UTC)
References
- ^ "Green fluorescent protein is a quantitative reporter of gene expression in individual eukaryotic cells". The FASEB Journal. March 2005. doi:10.1096/fj.04-3180fje. ISSN 0892-6638. PMC 1242169. PMID 15640280.
{{cite journal}}
: CS1 maint: unflagged free DOI (link) - ^ "Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222". Nature Structural Biology. Jan 2002. doi:10.1038/nsb739. ISSN 1072-8368. PMID 11740505.
Spamming Wikipedia
[edit]Biologicalworld.com has spammed wikipedia like no tomorrow. It is a site of only a few pages and a LOT of adsense. Not much information is given except for "protocols" which are not referenced, and cannot be trusted from a site of that quality.
check: Links from Wikipedia
The following have been cleaned up:
- en.wikipedia.org/wiki/Plasmid
- en.wikipedia.org/wiki/Gel_electrophoresis
- en.wikipedia.org/wiki/Green_fluorescent_protein
- en.wikipedia.org/wiki/Homology_(biology)
- en.wikipedia.org/wiki/HeLa
- en.wikipedia.org/wiki/Protease
- en.wikipedia.org/wiki/Restriction_enzyme
- en.wikipedia.org/wiki/Petri_dish
- en.wikipedia.org/wiki/Structural_domain
- en.wikipedia.org/wiki/Trypsin
- en.wikipedia.org/wiki/Oligonucleotide
- en.wikipedia.org/wiki/Transmission_electron_microscope
- en.wikipedia.org/wiki/Agar_plate
- en.wikipedia.org/wiki/Calcium_phosphate
- en.wikipedia.org/wiki/Disulfide_bond
- en.wikipedia.org/wiki/Denaturation_(biochemistry)
- en.wikipedia.org/wiki/DNA_ligase
- en.wikipedia.org/wiki/Wild_type
- en.wikipedia.org/wiki/Tissue_culture
- en.wikipedia.org/wiki/Transmission_electron_microscopy
- en.wikipedia.org/wiki/Reporter_gene
- en.wikipedia.org/wiki/Northern_blot
- en.wikipedia.org/wiki/Protein_engineering
- en.wikipedia.org/wiki/Sticky_end/blunt_end
- en.wikipedia.org/wiki/Taq_polymerase
- en.wikipedia.org/wiki/Protein_domain
- en.wikipedia.org/wiki/Coomassie
- en.wikipedia.org/wiki/Native_state
- en.wikipedia.org/wiki/Chinese_Hamster_Ovary_cell
- en.wikipedia.org/wiki/Peptidase
- en.wikipedia.org/wiki/Visking_tubing
- en.wikipedia.org/wiki/Streptavidin
- en.wikipedia.org/wiki/Microtiter_plate
- en.wikipedia.org/wiki/Subcloning
- en.wikipedia.org/wiki/Ion_exchange_chromatography
- en.wikipedia.org/wiki/Thermal_cycler
- en.wikipedia.org/wiki/Bovine_serum_albumin
- en.wikipedia.org/wiki/Phosphate_buffered_saline
- en.wikipedia.org/wiki/Glutathione_S-transferase
- en.wikipedia.org/wiki/HEPES
- en.wikipedia.org/wiki/Ortholog
- en.wikipedia.org/wiki/Proteases
- en.wikipedia.org/wiki/Salting_out
- en.wikipedia.org/wiki/Fetal_bovine_serum
- en.wikipedia.org/wiki/Proteolytic_enzyme
- en.wikipedia.org/wiki/DNA_end
- en.wikipedia.org/wiki/Supernatant
- en.wikipedia.org/wiki/ABTS
- en.wikipedia.org/wiki/Conserved_sequence
- en.wikipedia.org/wiki/Peptide_mass_fingerprinting
- en.wikipedia.org/wiki/Dithiothreitol
- en.wikipedia.org/wiki/Uranyl_acetate
and many more Sciencetalks (talk) 03:24, 4 January 2008 (UTC)
References
[edit]- Start-Class Molecular Biology articles
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