Unique molecular identifier

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Unique molecular identifiers (UMIs), or molecular barcodes (MBC) are short sequences or molecular "tags" added to DNA fragments in some next generation sequencing library preparation protocols to identify the input DNA molecule. They can be used to reduce errors and quantitative bias introduced by amplification. Applications include variant calling in ctDNA, gene expression in single-cell RNA-seq (scRNA-seq)[1][2] and haplotyping via linked reads.

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  1. ^ Kivioja T, Vähärautio A, Karlsson K, Bonke M, Enge M, Linnarsson S, Taipale J (2012). "Counting absolute numbers of molecules using unique molecular identifiers". Nat. Methods. 9 (1): 72–4. doi:10.1038/nmeth.1778. PMID 22101854.
  2. ^ Islam S, Zeisel A, Joost S, La Manno G, Zajac P, Kasper M, Lönnerberg P, Linnarsson S (2014). "Quantitative single-cell RNA-seq with unique molecular identifiers". Nat. Methods. 11 (2): 163–6. doi:10.1038/nmeth.2772. PMID 24363023.