User:Ceyockey/sandbox/PMID8496608

This is an experiment - to see how a pubmed abstract might be marked up using Wikipedia as a target.

PMID 8496608

Human intestinal intraepithelial lymphocytes are derived from a limited number of T cell clones that utilize multiple V beta T cell receptor genes

Blumberg RS, Yockey CE, Gross GG, Ebert EC, Balk SP

Intestinal intraepithelial lymphocytes (IEL) are a phenotypically distinct T cell population of unknown function. The majority of human intestinal IEL express the TCR-alpha beta, the CD8 accessory molecule, and the CD45RO Ag, suggesting that they are MHC class I-restricted memory T cells. Recent analyses of the TCR alpha- and beta-chains expressed by these cells have shown marked skewing toward one or several V region genes in individual donors and revealed the presence of clonally expanded cells. In addition, functional data has suggested that the MHC class I-like CD1 molecules may be the target ligands for some human intestinal IEL clones. This report examines in detail the TCR-beta repertoire of human jejunal IEL to determine what fraction of these cells are clonally expanded and to determine whether a particular subset of V beta genes are utilized by the clonally expanded cells. The results demonstrate that the majority of IEL are derived from the expansion of a relatively few T cell clones and that these clones can utilize a large number of different V beta genes. Oligoclonal expansion is also demonstrated among lamina propria lymphocytes (LPL), with overlapping but distinct clones detected in the LPL vs the IEL populations. These results indicate that most intestinal IEL-alpha beta, and a subpopulation of LPL, are specific for a limited number of Ag and place constraints on the possible roles played by IEL in the defense against diverse environmental pathogens or in the generation of oral tolerance.

Analysis

Missing and under-developed concepts

• Intestinal IEL, concept is mentioned in the IEL article, but without expansion
• human intestinal IEL vs. that of other species - concept does not come up in the IEL article
• TCR-alpha beta and the individual TCR chains - this is best addressed in the TCR article in the structural characteristics section, but functional differentiation is not addressed
• CD8 accessory molecule - this is actually poor wording in the abstract as CD8 is by definition a TCR 'accessory molecule' or co-receptor
• MHC class I-restricted memory T cells - in the memory T cell article, only MHC class II is mentioned and there is no content related to restriction to one or the other class
• V region - Wikipedia skews toward relating this exclusively to antibodies, when it is a more general concept across many molecule types