User:Hankwbass/Group 5
This page is for Group 5 - Genomics - to track their recommended changes and draft their work.
You should use the "Talk" page link above to offer comments on how you would improve your article. If your idea has already been suggested by another student, select "Edit" and leave a comment seconding their suggestion and leaving input of your own if there is something you'd like to add. Be sure to sign all your comments with 4 ~.
Once your group has come to consensus on 2-3 changes you'd like to propose, you can use the draft space below to draft your contribution.
Article draft
[edit]Citations: For both the Genomics and Recombinant DNA page, we decided that the citations need more work as the articles linked are giving either dead-links or are re-directing to pages that aren't related to the article. Below are the following edits we proposed to either remove or replace: (List the page/link number/link source and either declare removal or what we're changing it to. Sign after your citation so you can get the credit for it, I've went ahead and included some of what y'all had proposed with your user names so feel free to delete it and sign it with your name.) La15d (talk) 18:28, 20 April 2017 (UTC)
- "Kaiser O, Bartels D, Bekel T, Goesmann A, Kespohl S, Pühler A, Meyer F (Dec 2003). "Whole genome shotgun sequencing guided by bioinformatics pipelines--an optimized approach for an established technique" (PDF). Journal of Biotechnology. 106 (2–3): 121–33. doi:10.1016/j.jbiotec.2003.08.008. PMID 14651855. - Recommended by Res13k (feel free to edit the format of your source, delete this part and replace with your sign)
- "Donna U. Vogt and Mickey Parish. (1999) Food Biotechnology in the United States: Science, Regulation, and Issues." - Recommended by Abbystewart (talk) 01:16, 21 April 2017 (UTC)
- Recombinant DNA/Source 17/Fernandez, M.; Hosey, R. (2009). "Performance-enhancing drugs snare nonathletes, too". The Journal of family practice. 58 (1): 16–23. PMID 19141266./ Doesn't lead to article; it links to a publication database with the title of the article but you have to click around to eventually find the article. I suggest we redirect the link to the actual abstract page: http://www.mdedge.com/jfponline/article/63418/performance-enhancing-drugs-snare-nonathletes-too La15d (talk) 18:42, 20 April 2017 (UTC)
- Recombinant DNA/Source 6/ Russell, David W.; Sambrook, Joseph (2001). Molecular cloning: a laboratory manual. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory. ISBN 0-87969-576-5./ Although it is a book source, there is a full pdf of the article online. Suggest redirect to article page - http://www.cshlpress.com/pdf/sample/2013/MC4/MC4FM.pdf La15d (talk) 19:39, 20 April 2017 (UTC)
Recombinant DNA article introduction: There were many suggestions for revising the first three introductory paragraphs on this article. Most edits deal with syntax and the current colloquial phrasing of the article, but there was also a lot of research and information offered up that will help expand the article and provide more information. Below is a more finalized, compiled edit for these introductory paragraphs. It combines recommendations from the following editors (you guys can sign this!) and myself: Jackierodz518, AndrewBrowning, Tc16e, Res13k, Csf14c, ElizaBarrett, Austinjak, and Thokalath19.
"Recombinant DNA molecules are DNA molecules formed by laboratory methods of genetic recombination (such as molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in the genome. The construction of recombinant DNA is possible due to the universality of the genetic code.
Recombinant DNA is the general name for a piece of DNA that has been created in vitro by the combination of at least two DNA strands from different sources. Recombinant DNA molecules are sometimes called chimeric DNA, because they can be made of material from two difference species, like the mythical chimera (mythology). Bacterial plasmids are a popular form of cloning vector. To form a recombinant DNA molecule, the host vector and donor DNA both contain a specific palindromic sequence called a restriction site that attracts the same restriction enzyme. The enzyme cuts the strand into DNA fragments, each containing sticky (cohesive) or blunt ends. The host and donor ends are then bonded by another enzyme, DNA ligase, to form the new recombinant DNA molecule..[1][2][3]
The construction of recombinant DNA molecules may contain DNA sequences originating from any species. For example, plant DNA may be joined to bacterial DNA. Additionally, DNA sequences that do not occur in nature may be created by Oligonucleotide synthesis and incorporated into recombinant molecules. A multitude of DNA sequences may be created, and these DNA sequences may be introduced into a large range of living organisms." Abbystewart (talk) 18:40, 21 April 2017 (UTC)
Also, biopolymers should be mentioned in the 'Uses' section of the Recombinant DNA wiki page.
- "Biopolymers have properties that are useful to pharmaceutical, manufacturing, and food-processing industries. Synthetic polymers have been replaced with biological polymers using Recombinant DNA technologies, enhancing their structural and physical characteristics, decreasing manufacturing costs, and increase yields. Xanthan gum, Melanin, rubber, polyhydroxyalkanoates, and hyaluronic acid are examples of biopolymers produced using Recombinant DNA technology."[4]
By providing hyperlinks, excessive details about each biopolymer can be avoided - but these are important commercial products that are synthesized by recombinant microorganisms and should be mentioned. --Austinjak (talk) 14:45, 27 April 2017 (UTC)
- ^ Pingoud, A., & Jeltsch, A. (2001). Structure and function of type II restriction endonucleases. Nucleic Acids Research, 29(18), 3705-3727.
- ^ Doi, N., Kumadaki, S., Oishi, Y., Matsumura, N., & Yanagawa, H. (2004). In vitro selection of restriction endonucleases by in vitro compartmentalization. Nucleic Acids Research, 32(12), e95. doi:http://dx.doi.org/10.1093/nar/gnh096
- ^ Ryu, J., & Rowsell, E. (2008). Quick identification of type I restriction enzyme isoschizomers using newly developed pTypeI and reference plasmids. Nucleic Acids Research, 36(13), 1. doi:http://dx.doi.org/10.1093/nar/gkn056
- ^ Glick, Bernard R.; Pasternak, Jack J.; Patten, Cheryl L. (2010). Molecular Biotechnology: Principles and Applications of Recombinant DNA (4th Edition ed.). Washington, DC: ASM Press. pp. 535–545.
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