User:ManVhv/Sandbox/EBOV Proteins

The genome is non-segmented negative-sense RNA (nsnsRNA).

The gene order is 3′ – leader – NP – VP35 – VP40 – GP/sGP – VP30 – VP24 – L – trailer – 5′

• NP
• VP35
• VP40
• GP/sGP
• VP30
• VP24
• L - "Large" polymerase - complex with VP35 constitutes RNA-dependent RNA polymerase (RdRp)

• PMID 10073695

• Determined Nucletoide sequence of L encoding gene and 5' end of RNA.

• RNA has some folded structure, with self-complementary regions on 5' end, and 3' is complemetary to 5'.
• L protein has conserved amino acid sequences with other nsnsRNA viruses

• Conserved functionality is RNA-dependent RNA polymerase (RdRp)

• PMID 17218273

• VSV L protein is catalyst for 5'-end capping
• VSV mRNA is capped with GDP instead of GTP
• VSV L caps mRNA via "RNA:GDP polyribonucleotidyltransferase activity, in which a 5'-monophosphorylated viral mRNA-start sequence is transferred to GDP generated from GTP via a covalent enzyme-RNA intermediate"

	NP	VP35	VP40	GP/sGP	VP30	VP24	L
NP		nucleocapsid			nucleocapsid	nucleocapsid
VP35					RdRp		RdRp
VP40
GP/sGP
VP30						nucleocapsid	RdRp
VP24
L
EBOV dsRNA		X
EBOV mRNA							capping 5' with GDP
NPC-1				entry

• L-L and L-VP35 PMID 23582637