User:Qwuery/CODEHOP

WIP.

Consensus-Degenerate Hybrid Oligonucleotide Primer (CODEHOP) PCR primers derived from amino acid sequence motifs which are highly conserved between members of a protein family have proven to be highly effective in the identification and characterization of distantly related family members. CODEHOPs are designed from amino acid sequence motifs that are highly conserved within members of a gene family, and are used in PCR amplification to identify unknown related family members.

Only a very small fraction of the vast number of viral species belonging to the different virus families have been identified and characterized to date.

disease outbreaks caused by novel cross-species infections and/or subsequent virus recombination events have occurred [1]. Therefore, the development of tools for the detection of viruses, the characterization of their genomes and the study of their evolution, becomes important, not only for basic scientific study, but also for the protection of public health and the well-being of the plant and animal life that surrounds us.

We have utilized the CODEHOP technique to develop novel assays to detect previously unknown viral species by targeting sequence motifs within stable housekeeping genes that are evolutionarily conserved between different members of virus families.

[1] © 2005 Rose; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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