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Applications in florescence
Phycobiliprotein has many applications in fluorescent experiments due to its ability to participate in multi-color detection, as the fluorescent colors derived from it won't overlap and give low background noise [1]. This allows for high sensitivity and a wide range of uses for Fluorescent tags such as R- and B-Phycoerythrin and Allophycocyanin, all of which are made from phycobiliproteins[1]. One application is in Flow cytometry which measures and detects the different chemical and physical characteristics a large pool. This pool can include cells or particles that are in fluid. Cell sorting by fluorescence is another application, where live cells labeled with different fluorescent tags and can be sorted. Histochemistry can also apply this as it is used when trying to find chemicals that are related to different biological structures[2]. These are but a few examples of the many fluorescent applications that phycobiliproteins can provide.
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- ^ a b Manirafasha, Emmanuel; Ndikubwimana, Theoneste; Zeng, Xianhai; Lu, Yinghua; Jing, Keju (2016-05). "Phycobiliprotein: Potential microalgae derived pharmaceutical and biological reagent". Biochemical Engineering Journal. 109: 282–296. doi:10.1016/j.bej.2016.01.025. ISSN 1369-703X.
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(help) - ^ Li, Wenjun; Su, Hai-Nan; Pu, Yang; Chen, Jun; Liu, Lu-Ning; Liu, Qi; Qin, Song (2019-03). "Phycobiliproteins: Molecular structure, production, applications, and prospects". Biotechnology Advances. 37 (2): 340–353. doi:10.1016/j.biotechadv.2019.01.008. ISSN 0734-9750.
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