Buffered Charcoal Yeast Agar is a growth medium that is selected to grow Legionnaire's Bacterium. It contains L-Cystine amino acid and ferric pyrophosphate that assist in the growth of Legionnaire's species. The charcoal within the medium acts as a detoxicant because it decomposes hydrogen peroxide which is toxic to the Legionnaire's bacterium, the charcoal is also capable of changing the surface tension as well as collect carbon dioxide. The yeast extract in BCYE is the rich source of food(vitamins, nitrogen, and carbon) that the bacteria will use to grow. BCYE also has ACES buffer which maintains an optimal PH level for the bacteria to grow which is around 6.9. [1]
The Legionella bacteria that are smooth colorless to blue or grey will become more white and filamentous over time and appear green and yellow fluorescent under UV light. The colony surface is typically smooth but may look like it has strains that give it a fried egg type of appearance when looked at under a microscope.
BCYE plates should be stored in the dark at temperatures of 2-8 degree Celsius. Plates should not be frozen or overheated keep exposure to light minimal. BCYE along with most growth mediums should not be opened unless it is being used and should be at room temperature before inoculating anything onto the medium.
