User:Will-moore-dundee
Seed Cells
[edit]Seed Cells
[edit]Procedure
[edit]Warm PBS & Media to 37'C
[edit]Defrost Trypsin.
Wash cells in PBS
[edit]Rinse cells 3x in warm PBS.
Add trypsin: 5 ml
[edit]Default Value = 5Add trypsin, then remove and add another few ml. Remove and incubate, with shaking, till cells start to come off the dish.
Resuspend in media: 5 ml
[edit]Default Value = 10This will stop the trypin reaction.
Cell Line
[edit]Default Value = HeLa
Description
[edit]Notes about the cells:ie Are they stably transfected?Where they came from, etc.
Passage Number passages
[edit]The number of times the cells have been passaged since they started growing.
Plate format: 6-well plate
[edit]Default Value = 24-well plate