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Peer review for Blueboombox's "Fluorescence polarization immunoassay" article

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The article does a good job describing a biochemical technique used for rapid detection of antibodies or antigens. While the article has an appropriate structure, it is missing an info box, a template commonly seen in articles regarding biochemical techniques. Generally, the article flows well. It is clearly and precisely written and presents facts without bias. However, several sentences could be revised for better efficiency. For example, in the lead section, the sentence “It is a competitive homogenous... to be executed without…” would benefit without the phrase, “to be executed”. Moreover, the phrase “resulting in an altered intensity of plane-polarized light, which results in altered fluorescence polarization” could be rewritten as “altering the intensity of plane-polarized light, resulting in an altered fluorescence polarization”. Other than that, while the lead section provides a concise summary of the topic, it does not entirely prepare readers for the detail in the subsequent sections.


Lemontree96 (talk) 01:15, 14 April 2017 (UTC)[reply]

Peer Review

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In first lines of the introduction, it would be useful to explain what a competitive homogenous assay is, or at least describe it in terms better for a lay-person. If not that, then perhaps just link that phrase to a related WP article so that people (like myself) who are unfamiliar with the phrase can understand the basic concept behind the assay. While the second paragraph is well worded and understandable from a lay standpoint, there are no citations which are called for with an explanation of that length.

The History section is good to have, but perhaps be a bit more detailed about what Perrin contributed to the field. IT is safe to say that what he created is still used, but it would be useful to the reader to know what that process entails.

A "see also" page would be useful for readers looking to expand their knowledge on the subject: perhaps to some of the articles already linked in fluorescence anisotropy or immunoassay articles.

On a grammatical note, there are a number of issues with commas in the article, like "Fluorescence polarization immunoassays employ a fluorophore bound antigen that when bound to the antibody of interest, will increase fluorescence polarization" which should have a comma after "then", and "FPIA is a competitive homogenous assay, that is comprised of a simple prepare and read method, without the requirement of separation or washing steps" which should have no commas.

Otherwise, this is a very strong article, especially for having been built from the ground up. — Preceding unsigned comment added by 2spooky (talkcontribs) 20:52, 14 April 2017 (UTC)[reply]