Water blue

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For the butterfly with this common name, see Cacyreus tespis.
Water blue
Water blue2.png
3D model (Jmol)
ECHA InfoCard 100.044.634
Molar mass 737.72374 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Water blue, also known as aniline blue, Acid blue 22, Soluble Blue 3M, Marine Blue V, or C.I. 42755, is a chemical compound used as a stain in histology. Water blue stains collagen blue in tissue sections. It is soluble in water and slightly soluble in ethanol.

Water blue is also available in mixture with methyl blue, under name Aniline Blue WS, Aniline blue, China blue, or Soluble blue.

It can be used in the Mallory's trichrome stain of connective tissue and Gömöri trichrome stain of muscle tissue. It is used in differential staining.[1]


  1. ^ Nowicki, Marcin; et al. (15 May 2013), A simple dual stain for detailed investigations of plant-fungal pathogen interactions, Vegetable Crops Research bulleting, InHort & Versita, doi:10.2478/v10032-012-0016-z, retrieved 2013-05-24