Integrin-linked kinase (ILK) is a 59kDa protein originally identified while conducting a yeast-two hybrid screen with integrin β1 as the bait protein (Hannigan et al., 1996). Since its discovery, ILK has been associated with multiple cellular functions including cell migration, cell proliferation, cell-adhesions, and signal transduction.
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene was initially described to encode a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. Recent results showed that ILK contains 5 ankyrin-like repeats, and that the C-terminal kinase domain is actually a pseudo-kinase with adaptor function.
^Zhang, Yongjun; Chen Ka; Guo Lida; Wu Chuanyue (October 2002). "Characterization of PINCH-2, a new focal adhesion protein that regulates the PINCH-1-ILK interaction, cell spreading, and migration". J. Biol. Chem.277 (41): 38328–38. doi:10.1074/jbc.M205576200. PMID12167643.
^Barry, Fiona A; Gibbins Jonathan M (April 2002). "Protein kinase B is regulated in platelets by the collagen receptor glycoprotein VI". J. Biol. Chem.277 (15): 12874–8. doi:10.1074/jbc.M200482200. PMID11825911.
^Persad, S; Attwell S; Gray V; Mawji N; Deng J T; Leung D; Yan J; Sanghera J; Walsh M P; Dedhar S (July 2001). "Regulation of protein kinase B/Akt-serine 473 phosphorylation by integrin-linked kinase: critical roles for kinase activity and amino acids arginine 211 and serine 343". J. Biol. Chem.276 (29): 27462–9. doi:10.1074/jbc.M102940200. PMID11313365.
Hannigan GE, Leung-Hagesteijn C, Fitz-Gibbon L, et al. (1996). "Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase". Nature379 (6560): 91–6. doi:10.1038/379091a0. PMID8538749.
Hannigan GE, Bayani J, Weksberg R, et al. (1997). "Mapping of the gene encoding the integrin-linked kinase, ILK, to human chromosome 11p15.5-p15.4". Genomics42 (1): 177–9. doi:10.1006/geno.1997.4719. PMID9177792.
Li F, Liu J, Mayne R, Wu C (1997). "Identification and characterization of a mouse protein kinase that is highly homologous to human integrin-linked kinase". Biochim. Biophys. Acta1358 (3): 215–20. doi:10.1016/S0167-4889(97)00089-X. PMID9366252.
Chung DH, Lee JI, Kook MC, et al. (1998). "ILK (beta1-integrin-linked protein kinase): a novel immunohistochemical marker for Ewing's sarcoma and primitive neuroectodermal tumour". Virchows Arch.433 (2): 113–7. doi:10.1007/s004280050225. PMID9737788.
Feng J, Ito M, Ichikawa K, et al. (2000). "Inhibitory phosphorylation site for Rho-associated kinase on smooth muscle myosin phosphatase". J. Biol. Chem.274 (52): 37385–90. doi:10.1074/jbc.274.52.37385. PMID10601309.
Janji B, Melchior C, Vallar L, Kieffer N (2000). "Cloning of an isoform of integrin-linked kinase (ILK) that is upregulated in HT-144 melanoma cells following TGF-beta1 stimulation". Oncogene19 (27): 3069–77. doi:10.1038/sj.onc.1203640. PMID10871859.
Velyvis A, Yang Y, Wu C, Qin J (2001). "Solution structure of the focal adhesion adaptor PINCH LIM1 domain and characterization of its interaction with the integrin-linked kinase ankyrin repeat domain". J. Biol. Chem.276 (7): 4932–9. doi:10.1074/jbc.M007632200. PMID11078733.
Matsumoto M, Ogawa W, Hino Y, et al. (2001). "Inhibition of insulin-induced activation of Akt by a kinase-deficient mutant of the epsilon isozyme of protein kinase C". J. Biol. Chem.276 (17): 14400–6. doi:10.1074/jbc.M011093200. PMID11278835.
Nikolopoulos SN, Turner CE (2001). "Integrin-linked kinase (ILK) binding to paxillin LD1 motif regulates ILK localization to focal adhesions". J. Biol. Chem.276 (26): 23499–505. doi:10.1074/jbc.M102163200. PMID11304546.
Persad S, Attwell S, Gray V, et al. (2001). "Regulation of protein kinase B/Akt-serine 473 phosphorylation by integrin-linked kinase: critical roles for kinase activity and amino acids arginine 211 and serine 343". J. Biol. Chem.276 (29): 27462–9. doi:10.1074/jbc.M102940200. PMID11313365.