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Replication factor C (activator 1) 1, 145kDa
Protein RFC1 PDB 2EBU.png
Rendering based on PDB 2EBU.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols RFC1 ; A1; MHCBFB; PO-GA; RECC1; RFC; RFC140
External IDs OMIM102579 MGI97891 HomoloGene2187 GeneCards: RFC1 Gene
RNA expression pattern
PBB GE RFC1 208021 s at tn.png
PBB GE RFC1 209085 x at tn.png
More reference expression data
Species Human Mouse
Entrez 5981 19687
Ensembl ENSG00000035928 ENSMUSG00000029191
UniProt P35251 P35601
RefSeq (mRNA) NM_001204747 NM_011258
RefSeq (protein) NP_001191676 NP_035388
Location (UCSC) Chr 4:
39.29 – 39.37 Mb
Chr 5:
65.26 – 65.34 Mb
PubMed search [1] [2]

Replication factor C subunit 1 is a protein that in humans is encoded by the RFC1 gene.[1][2]


The protein encoded by this gene is the large subunit of replication factor C, which is a five subunit DNA polymerase accessory protein. Replication factor C is a DNA-dependent ATPase that is required for eukaryotic DNA replication and repair. The protein acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It also may have a role in telomere stability.[2]


RFC1 has been shown to interact with:


  1. ^ Luckow B, Bunz F, Stillman B, Lichter P, Schütz G (Mar 1994). "Cloning, expression, and chromosomal localization of the 140-kilodalton subunit of replication factor C from mice and humans". Mol Cell Biol 14 (3): 1626–34. PMC 358521. PMID 8114700. 
  2. ^ a b "Entrez Gene: RFC1 replication factor C (activator 1) 1, 145kDa". 
  3. ^ a b Maruyama T, Farina A, Dey A, Cheong J, Bermudez VP, Tamura T, Sciortino S, Shuman J, Hurwitz J, Ozato K (Sep 2002). "A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase". Mol. Cell. Biol. 22 (18): 6509–20. doi:10.1128/mcb.22.18.6509-6520.2002. PMC 135621. PMID 12192049. 
  4. ^ Anderson LA, Perkins ND (Aug 2002). "The large subunit of replication factor C interacts with the histone deacetylase, HDAC1". J. Biol. Chem. 277 (33): 29550–4. doi:10.1074/jbc.M200513200. PMID 12045192. 
  5. ^ Fotedar R, Mossi R, Fitzgerald P, Rousselle T, Maga G, Brickner H, Messier H, Kasibhatla S, Hübscher U, Fotedar A (Aug 1996). "A conserved domain of the large subunit of replication factor C binds PCNA and acts like a dominant negative inhibitor of DNA replication in mammalian cells". EMBO J. 15 (16): 4423–33. PMC 452166. PMID 8861969. 
  6. ^ Mossi R, Jónsson ZO, Allen BL, Hardin SH, Hübscher U (Jan 1997). "Replication factor C interacts with the C-terminal side of proliferating cell nuclear antigen". J. Biol. Chem. 272 (3): 1769–76. doi:10.1074/jbc.272.3.1769. PMID 8999859. 
  7. ^ van der Kuip H, Carius B, Haque SJ, Williams BR, Huber C, Fischer T (Apr 1999). "The DNA-binding subunit p140 of replication factor C is upregulated in cycling cells and associates with G1 phase cell cycle regulatory proteins". J. Mol. Med. 77 (4): 386–92. doi:10.1007/s001090050365. PMID 10353443. 
  8. ^ Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T (Oct 2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". J. Biol. Chem. 277 (43): 40362–7. doi:10.1074/jbc.M206194200. PMID 12171929. 
  9. ^ Anderson LA, Perkins ND (Jan 2003). "Regulation of RelA (p65) function by the large subunit of replication factor C". Mol. Cell. Biol. 23 (2): 721–32. doi:10.1128/mcb.23.2.721-732.2003. PMC 151544. PMID 12509469. 
  10. ^ Ellison V, Stillman B (Mar 1998). "Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity". J. Biol. Chem. 273 (10): 5979–87. doi:10.1074/jbc.273.10.5979. PMID 9488738. 
  11. ^ Uhlmann F, Cai J, Flores-Rozas H, Dean FB, Finkelstein J, O'Donnell M, Hurwitz J (Jun 1996). "In vitro reconstitution of human replication factor C from its five subunits". Proc. Natl. Acad. Sci. U.S.A. 93 (13): 6521–6. doi:10.1073/pnas.93.13.6521. PMC 39056. PMID 8692848. 
  12. ^ Tomida J, Masuda Y, Hiroaki H, Ishikawa T, Song I, Tsurimoto T, Tateishi S, Shiomi T, Kamei Y, Kim J, Kamiya K, Vaziri C, Ohmori H, Todo T (Apr 2008). "DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex". J. Biol. Chem. 283 (14): 9071–9. doi:10.1074/jbc.M709835200. PMC 2431014. PMID 18245774. 

Further reading[edit]