Pulmonary surfactant-associated protein A1

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Surfactant protein A1
Identifiers
Symbols SFTPA1 ; COLEC4; PSAP; PSP-A; PSPA; SFTP1; SFTPA1B; SP-A; SP-A1; SPA; SPA1
External IDs OMIM178630 HomoloGene3946 GeneCards: SFTPA1 Gene
Orthologs
Species Human Mouse
Entrez 653509 20387
Ensembl ENSG00000122852 ENSMUSG00000021789
UniProt Q8IWL2 P35242
RefSeq (mRNA) NM_001093770 NM_023134
RefSeq (protein) NP_001087239 NP_075623
Location (UCSC) Chr 10:
81.37 – 81.38 Mb
Chr 14:
41.13 – 41.14 Mb
PubMed search [1] [2]

Pulmonary surfactant-associated protein A1 (PSP-A), also known as surfactant protein A1 (SFTPA1) is a protein that in humans is encoded by the SFTPA1 gene.[1][2]

Summary[edit]

The protein encoded by this gene (SP-A1) is primarily synthesized in lung alveolar type II cells (see type II pneumocyte), as part of a complex of lipids and proteins known as pulmonary surfactant. The function of this complex is to reduce surface tension in the alveolus and prevent collapse during expiration. The protein component of surfactant helps in the modulation of the innate immune response, and inflammatory processes.[3]

Alveolar sac region of the lung - TEM

SP-A1 is a member of a subfamily of C-type lectins called collectins. Together with SP-A2 (see SFTPA2), they are the most abundant proteins of pulmonary surfactant. SP-A1 binds to the carbohydrates found in the surface of several microorganisms and helps in the defense against respiratory pathogens.[4][5][6]

Surfactant homeostasis is critical for breathing (and thus survival) in the prematurely born infant, but also for maintaining lung health, and normal lung function throughout life. Quantitative and/or qualitative alterations in surfactant composition and/or function are associated with respiratory diseases.[7][8][9][10]


SFTPA1 expression[edit]

The lung is the main site of SFTPA1 synthesis, but SFTPA1 mRNA expression has also been detected in the trachea, prostate, pancreas, thymus, colon, eye, salivary gland and other tissues.[11] Using specific monoclonal antibodies for Surfactant protein A, the protein can be detected in lung alveolar type II pneumocytes, Clara cells, and alveolar macrophages, but no extrapulmonary SP-A immunoreactivity was observed.[11]

Gene[edit]

SFTPA1 is located in the long arm of chromosome 10, close to SFTPA2. The SFTPA1 gene is 4505 base pairs in length, and 94% similar to SFTPA2. The structure of SFTPA1 consists of four coding exons (I-IV), and several 5'UTR untranslated exons (A, B, B’, C, C’,D, D’).[12][13] The expression of SFTPA1 is regulated by cellular factors including proteins, small RNAs (microRNAs), glucocorticoids, etc. Its expression is also regulated by epigenetic and environmental factors.[14]

Differences in the SFTPA1 gene sequence at the coding region determine SP-A genetic variants or haplotypes among individuals.[13] More than 30 variants have been identified and characterized for SFTPA1 (and SFTPA2) in the population. SFTPA1 variants result from nucleotide changes in the codons of amino acids 19, 50, 62, 133, and 219. Two of these do not modify the SP-A1 protein sequence (amino acids 62 and 133), whereas the rest result in amino acid substitutions (amino acid 19, 50, 133, and 219). Four SP-A1 variants (6A, 6A2, 6A3, 6A4) are in higher frequency in the general population. The most frequently found variant is 6A2.[15][16]

Structure[edit]

Surfactant protein A (SP-A) is a protein of 248 amino acids usually found in large oligomeric structures. The mature SP-A1 monomer is a 35kDa protein that differs from SP-A2 in four amino acids at the coding region. The structure of SP-A1 monomers consists of four domains: an N-terminal, a collagen-like domain, a neck region, and a carbohydrate recognition domain. The C-terminal carbohydrate recognition domain (CRD) allows binding to various types of microorganisms and molecules.[15][16] The amino acid differences that distinguish between SP-A1 and SP-A2 genes and between their corresponding variants are located at the collagen-like domain. The amino acid differences that distinguish among SFTPA1 variants are located both at the carbohydrate recognition and the collagen-like domains.[15][17]

SP-A1 monomers group with other SP-A1 or SP-A2 monomers in trimeric structural subunits of 105kDa. Six of these structures group in 630 kDa structures that resemble flower bouquets. These oligomers contain a total of eighteen SP-A1 and/or SP-A2 monomers.[15]

Functions[edit]

Innate immunity[edit]

The role of SFTPA1 in innate immunity has been extensively studied. SP-A has the ability to bind and agglutinate bacteria, fungi, viruses, and other non-biological antigens. Some of the functions by which both SFTPA1 and SFTPA2 contribute to innate immunity include:

Environmental insults such as air pollution, and exposure to high concentrations of ozone and particulate matter can affect SP-A expression and function, via mechanisms that involve epigenetic regulation of SFTPA1 expression.[14]

Clinical significance[edit]

Deficiency in SP-A levels is associated with infant respiratory distress syndrome in prematurely born infants with developmental insufficiency of surfactant production and structural immaturity in the lungs.[18]

SFTPA1 genetic variants, SNPs, haplotypes, and other genetic variations have been associated with acute and chronic lung disease in several populations of neonates, children, and adults.[7] Genetic variations in SFTPA1 have been associated with susceptibility to idiopathic pulmonary fibrosis, a lung disease characterized by shortness of breath, pulmonary infiltrates and inflammation that results in acute lung injury with subsequent scarring of lung tissue.[19] Genetic variations in SFTPA1 are also a cause of susceptibility to respiratory distress syndrome in premature infants, a lung disease characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits "surfactant protein A1". . The ratio of SP-A1 to total SP-A has been correlated with lung disease (e.g. asthma, cystic fibrosis) and aging.[20][21] Methylation of SFTPA1 promoter sequences has also been found in lung cancer tissue.[22][23]

SFTPA1 mRNA transcript variants[edit]

Variant id 5’UTR splice Coding 3’UTR sequence GenBank id
AD'6A AD’ 6A 6A HQ021433
AD'6A2 AD’ 6A2 6A2 HQ021434
AD'6A3 AD’ 6A3 6A3 HQ021435
AD'6A4 AD’ 6A4 6A4 HQ021436
AB'D'6A AB'D’ 6A 6A JX502764
AB'D'6A2 AB'D’ 6A2 6A2 HQ021437
AB'D'6A3 AB'D’ 6A3 6A3 HQ021438
AB'D'6A4 AB'D’ 6A4 6A4 HQ021439
ACD'6A ACD’ 6A 6A JX502765
ACD'6A2 ACD’ 6A2 6A2 HQ021440
ACD'6A3 ACD’ 6A3 6A3 HQ021441
ACD'6A4 ACD’ 6A4 6A4 HQ021442
SFTPA1 variant 1 AB'D’ 6A3 6A3 NM_005411.4
SFTPA1 variant 2 ACD’ 6A3 6A3 NM_001093770.2
SFTPA1 variant 3 ABD’ 6A3 6A3 NM_001164644.1
SFTPA1 variant 4 AD’ 6A3 6A3 NM_001164647.1
SFTPA1 variant 5 ACD’ 6A3 (truncated) 6A3 NM_001164645.1
SFTPA1 variant 6 AB'D’ 6A3 (truncated) 6A3 NM_001164646.1

Gene regulation[edit]

Gene expression of SFTPA1 is regulated at different levels including gene transcription, post-transcriptional processing, stability and translation (biology) of mature mRNA.[2] One of the important features of human surfactant protein A mRNAs is that they have a variable five prime untranslated region (5’UTR) generated from splicing variation of exons A, B, C, and D.[24][25] At least 10 forms of human SFTPA1 and SFTPA2 5’UTRs have been identified that differ in nucleotide sequence, length, and relative amount.[26] Specific SFTPA1 or SFTPA2 5’UTRs have also been characterized. Some SFTPA1 specific 5’UTRs include exons B’ or C. These two exons contain upstream AUGs (uAUGs) that can potentially act as sites for translation initiation (see eukaryotic translation), affecting protein translation and SFTPA1 relative content. The majority of SFTPA1 transcripts lack exon B, a sequence implicated in transcription and translation enhancement, indicating a differential regulation of SFTPA1 and SFTPA2 expression.[27] The AD’ form is the most represented among SFTPA1 transcripts (81%),[26] and experimental work has shown that this sequence can stabilize mRNA and enhance translation, but the mechanisms implicated in this regulation are still under investigation.[28][29][30] While differences at the 5’UTR are shown to regulate both transcription and translation,[27] polymorphisms at the 3’UTR of SP-A1 variants are shown to primarily, differentially affect translation efficiency[29] via mechanisms that involve binding of proteins[31] and/or [microRNAs].[29] The impact of this regulation on SFTPA1 and SFTPA2 protein levels may contribute to individual differences in susceptibility to lung disease.[20][21] Environmental insults and pollutants also affect SFTPA1 expression. Exposure of lung cells to particulate matter affects splicing of 5’UTR exons of SFTPA1 transcripts. Pollutants and viral infections also affect SFTPA1 translation mechanisms (see eukaryotic translation, translation (biology)).[28][32]

See also[edit]

References[edit]

  1. ^ "Entrez Gene: Surfactant protein A1". 
  2. ^ a b Silveyra P, Floros J (December 2013). "Genetic complexity of the human surfactant-associated proteins SP-A1 and SP-A2". Gene 531 (2): 126–32. doi:10.1016/j.gene.2012.09.111. PMC 3570704. PMID 23069847. 
  3. ^ Perez-Gil J, Weaver TE (June 2010). "Pulmonary surfactant pathophysiology: current models and open questions". Physiology (Bethesda) 25 (3): 132–41. doi:10.1152/physiol.00006.2010. PMID 20551227. 
  4. ^ Crouch EC (August 1998). "Collectins and pulmonary host defense". Am. J. Respir. Cell Mol. Biol. 19 (2): 177–201. doi:10.1165/ajrcmb.19.2.140. PMID 9698590. 
  5. ^ Crouch E, Hartshorn K, Ofek I (February 2000). "Collectins and pulmonary innate immunity". Immunol. Rev. 173: 52–65. doi:10.1034/j.1600-065x.2000.917311.x. PMID 10719667. 
  6. ^ Phelps DS (2001). "Surfactant regulation of host defense function in the lung: a question of balance". Pediatr Pathol Mol Med 20 (4): 269–92. doi:10.1080/15513810109168822. PMID 11486734. 
  7. ^ a b Silveyra P, Floros J (2012). "Genetic variant associations of human SP-A and SP-D with acute and chronic lung injury". Front. Biosci. 17: 407–29. doi:10.2741/3935. PMID 22201752. 
  8. ^ Floros J, Kala P (1998). "Surfactant proteins: molecular genetics of neonatal pulmonary diseases". Annu. Rev. Physiol. 60: 365–84. doi:10.1146/annurev.physiol.60.1.365. PMID 9558469. 
  9. ^ Floros J, Wang G (May 2001). "A point of view: quantitative and qualitative imbalance in disease pathogenesis; pulmonary surfactant protein A genetic variants as a model". Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 129 (1): 295–303. doi:10.1016/S1095-6433(01)00325-7. PMID 11369553. 
  10. ^ Whitsett JA, Wert SE, Weaver TE (2010). "Alveolar surfactant homeostasis and the pathogenesis of pulmonary disease". Annu. Rev. Med. 61: 105–19. doi:10.1146/annurev.med.60.041807.123500. PMID 19824815. 
  11. ^ a b Madsen, J.; Tornøe, I.; Nielsen, O.; Koch, C.; Steinhilber, W.; Holmskov, U. (2003). "Expression and Localization of Lung Surfactant Protein a in Human Tissues". American Journal of Respiratory Cell and Molecular Biology 29 (5): 591–597. doi:10.1165/rcmb.2002-0274OC. PMID 12777246.  edit
  12. ^ Floros J, Hoover RR (November 1998). "Genetics of the hydrophilic surfactant proteins A and D". Biochim. Biophys. Acta 1408 (2-3): 312–22. doi:10.1016/S0925-4439(98)00077-5. PMID 9813381. 
  13. ^ a b DiAngelo S, Lin Z, Wang G, Phillips S, Ramet M, Luo J, Floros J (December 1999). "Novel, non-radioactive, simple and multiplex PCR-cRFLP methods for genotyping human SP-A and SP-D marker alleles". Dis. Markers 15 (4): 269–81. doi:10.1155/1999/961430. PMID 10689550. 
  14. ^ a b Silveyra P, Floros J (2012). "Air pollution and epigenetics: effects on SP-A and innate host defence in the lung". Swiss Med Wkly 142: w13579. doi:10.4414/smw.2012.13579. PMC 3601480. PMID 22553125. 
  15. ^ a b c d Floros J, Wang G, Mikerov AN (2009). "Genetic complexity of the human innate host defense molecules, surfactant protein A1 (SP-A1) and SP-A2--impact on function". Crit. Rev. Eukaryot. Gene Expr. 19 (2): 125–37. doi:10.1615/critreveukargeneexpr.v19.i2.30. PMC 2967201. PMID 19392648. 
  16. ^ a b "ingentaconnect Genetic Diversity of Human SP-A, a Molecule with Innate host Defe...". 
  17. ^ Wang G, Myers C, Mikerov A, Floros J (July 2007). "Effect of cysteine 85 on biochemical properties and biological function of human surfactant protein A variants". Biochemistry 46 (28): 8425–35. doi:10.1021/bi7004569. PMC 2531219. PMID 17580966. 
  18. ^ deMello DE, Heyman S, Phelps DS, Floros J (May 1993). "Immunogold localization of SP-A in lungs of infants dying from respiratory distress syndrome". Am. J. Pathol. 142 (5): 1631–40. PMC 1886897. PMID 8494055. 
  19. ^ Selman M, King TE, Pardo A (January 2001). "Idiopathic pulmonary fibrosis: prevailing and evolving hypotheses about its pathogenesis and implications for therapy". Ann. Intern. Med. 134 (2): 136–51. doi:10.7326/0003-4819-134-2-200101160-00015. PMID 11177318. 
  20. ^ a b Tagaram HR, Wang G, Umstead TM, Mikerov AN, Thomas NJ, Graff GR, Hess JC, Thomassen MJ, Kavuru MS, Phelps DS, Floros J (May 2007). "Characterization of a human surfactant protein A1 (SP-A1) gene-specific antibody; SP-A1 content variation among individuals of varying age and pulmonary health". Am. J. Physiol. Lung Cell Mol. Physiol. 292 (5): L1052–63. doi:10.1152/ajplung.00249.2006. PMID 17189324. 
  21. ^ a b Wang Y, Voelker DR, Lugogo NL, Wang G, Floros J, Ingram JL, Chu HW, Church TD, Kandasamy P, Fertel D, Wright JR, Kraft M (October 2011). "Surfactant protein A is defective in abrogating inflammation in asthma". Am. J. Physiol. Lung Cell Mol. Physiol. 301 (4): L598–606. doi:10.1152/ajplung.00381.2010. PMC 3191759. PMID 21784968. 
  22. ^ Vaid M, Floros J (January 2009). "Surfactant protein DNA methylation: a new entrant in the field of lung cancer diagnostics? (Review)". Oncol. Rep. 21 (1): 3–11. PMC 2899699. PMID 19082436. 
  23. ^ Lin Z, Thomas NJ, Bibikova M, Seifart C, Wang Y, Guo X, Wang G, Vollmer E, Goldmann T, Garcia EW, Zhou L, Fan JB, Floros J (July 2007). "DNA methylation markers of surfactant proteins in lung cancer". Int. J. Oncol. 31 (1): 181–91. doi:10.3892/ijo.31.1.181. PMID 17549420. 
  24. ^ Karinch AM, Deiter G, Ballard PL, Floros J (June 1998). "Regulation of expression of human SP-A1 and SP-A2 genes in fetal lung explant culture". Biochim. Biophys. Acta 1398 (2): 192–202. doi:10.1016/S0167-4781(98)00047-5. PMID 9689918. 
  25. ^ Karinch AM, Floros J (April 1995). "Translation in vivo of 5' untranslated-region splice variants of human surfactant protein-A". Biochem. J. 307 (2): 327–30. PMC 1136651. PMID 7733864. 
  26. ^ a b Karinch AM, Floros J (January 1995). "5' splicing and allelic variants of the human pulmonary surfactant protein A genes". Am. J. Respir. Cell Mol. Biol. 12 (1): 77–88. doi:10.1165/ajrcmb.12.1.7811473. PMID 7811473. 
  27. ^ a b Silveyra P, Raval M, Simmons B, Diangelo S, Wang G, Floros J (November 2011). "The untranslated exon B of human surfactant protein A2 mRNAs is an enhancer for transcription and translation". Am. J. Physiol. Lung Cell Mol. Physiol. 301 (5): L795–803. doi:10.1152/ajplung.00439.2010. PMC 3290452. PMID 21840962. 
  28. ^ a b Wang G, Guo X, Silveyra P, Kimball SR, Floros J (April 2009). "Cap-independent translation of human SP-A 5'-UTR variants: a double-loop structure and cis-element contribution". Am. J. Physiol. Lung Cell Mol. Physiol. 296 (4): L635–47. doi:10.1152/ajplung.90508.2008. PMC 2670766. PMID 19181744. 
  29. ^ a b c Silveyra P, Wang G, Floros J (October 2010). "Human SP-A1 (SFTPA1) variant-specific 3' UTRs and poly(A) tail differentially affect the in vitro translation of a reporter gene". Am. J. Physiol. Lung Cell Mol. Physiol. 299 (4): L523–34. doi:10.1152/ajplung.00113.2010. PMC 2957414. PMID 20693318. 
  30. ^ Wang G, Guo X, Floros J (September 2005). "Differences in the translation efficiency and mRNA stability mediated by 5'-UTR splice variants of human SP-A1 and SP-A2 genes". Am. J. Physiol. Lung Cell Mol. Physiol. 289 (3): L497–508. doi:10.1152/ajplung.00100.2005. PMID 15894557. 
  31. ^ Wang G, Guo X, Floros J (May 2003). "Human SP-A 3'-UTR variants mediate differential gene expression in basal levels and in response to dexamethasone". Am. J. Physiol. Lung Cell Mol. Physiol. 284 (5): L738–48. doi:10.1152/ajplung.00375.2002. PMID 12676764. 
  32. ^ Bruce SR, Atkins CL, Colasurdo GN, Alcorn JL (October 2009). "Respiratory syncytial virus infection alters surfactant protein A expression in human pulmonary epithelial cells by reducing translation efficiency". Am. J. Physiol. Lung Cell Mol. Physiol. 297 (4): L559–67. doi:10.1152/ajplung.90507.2008. PMC 2770795. PMID 19525387. 

Further reading[edit]

  • Lu J (1997). "Collectins: collectors of microorganisms for the innate immune system.". BioEssays 19 (6): 509–18. doi:10.1002/bies.950190610. PMID 9204768. 
  • Floros J, Hoover RR (1999). "Genetics of the hydrophilic surfactant proteins A and D.". Biochim. Biophys. Acta 1408 (2-3): 312–22. doi:10.1016/S0925-4439(98)00077-5. PMID 9813381. 
  • Khubchandani KR, Snyder JM (2001). "Surfactant protein A (SP-A): the alveolus and beyond.". FASEB J. 15 (1): 59–69. doi:10.1096/fj.00-0318rev. PMID 11149893. 
  • Katyal SL, Singh G, Locker J (1992). "Characterization of a second human pulmonary surfactant-associated protein SP-A gene.". Am. J. Respir. Cell Mol. Biol. 6 (4): 446–52. doi:10.1165/ajrcmb/6.4.446. PMID 1372511. 
  • Childs RA, Wright JR, Ross GF, Yuen CT, Lawson AM, Chai W, Drickamer K, Feizi T (1992). "Specificity of lung surfactant protein SP-A for both the carbohydrate and the lipid moieties of certain neutral glycolipids.". J. Biol. Chem. 267 (14): 9972–9. PMID 1577827. 
  • Endo H, Oka T (1991). "An immunohistochemical study of bronchial cells producing surfactant protein A in the developing human fetal lung.". Early Hum. Dev. 25 (3): 149–56. doi:10.1016/0378-3782(91)90111-F. PMID 1935736. 
  • Voss T, Melchers K, Scheirle G, Schäfer KP (1991). "Structural comparison of recombinant pulmonary surfactant protein SP-A derived from two human coding sequences: implications for the chain composition of natural human SP-A.". Am. J. Respir. Cell Mol. Biol. 4 (1): 88–94. doi:10.1165/ajrcmb/4.1.88. PMID 1986781. 
  • Haagsman HP, White RT, Schilling J, Lau K, Benson BJ, Golden J, Hawgood S, Clements JA (1990). "Studies of the structure of lung surfactant protein SP-A.". Am. J. Physiol. 257 (6 Pt 1): L421–9. PMID 2610270. 
  • Fisher JH, Kao FT, Jones C, White RT, Benson BJ, Mason RJ (1987). "The coding sequence for the 32,000-dalton pulmonary surfactant-associated protein A is located on chromosome 10 and identifies two separate restriction-fragment-length polymorphisms.". Am. J. Hum. Genet. 40 (6): 503–11. PMC 1684155. PMID 2884868. 
  • White RT, Damm D, Miller J, Spratt K, Schilling J, Hawgood S, Benson B, Cordell B (1985). "Isolation and characterization of the human pulmonary surfactant apoprotein gene.". Nature 317 (6035): 361–3. doi:10.1038/317361a0. PMID 2995821. 
  • Floros J, Steinbrink R, Jacobs K, Phelps D, Kriz R, Recny M, Sultzman L, Jones S, Taeusch HW, Frank HA (1986). "Isolation and characterization of cDNA clones for the 35-kDa pulmonary surfactant-associated protein.". J. Biol. Chem. 261 (19): 9029–33. PMID 3755136. 
  • Schaeffer E, Guillou F, Part D, Zakin MM (1993). "A different combination of transcription factors modulates the expression of the human transferrin promoter in liver and Sertoli cells.". J. Biol. Chem. 268 (31): 23399–408. PMID 8226864. 
  • Khoor A, Gray ME, Hull WM, Whitsett JA, Stahlman MT (1993). "Developmental expression of SP-A and SP-A mRNA in the proximal and distal respiratory epithelium in the human fetus and newborn.". J. Histochem. Cytochem. 41 (9): 1311–9. doi:10.1177/41.9.8354874. PMID 8354874. 
  • Strayer DS, Yang S, Jerng HH (1993). "Surfactant protein A-binding proteins. Characterization and structures.". J. Biol. Chem. 268 (25): 18679–84. PMID 8360162. 
  • Kölble K, Lu J, Mole SE, Kaluz S, Reid KB (1993). "Assignment of the human pulmonary surfactant protein D gene (SFTP4) to 10q22-q23 close to the surfactant protein A gene cluster.". Genomics 17 (2): 294–8. doi:10.1006/geno.1993.1324. PMID 8406480. 
  • deMello DE, Heyman S, Phelps DS, Floros J (1993). "Immunogold localization of SP-A in lungs of infants dying from respiratory distress syndrome.". Am. J. Pathol. 142 (5): 1631–40. PMC 1886897. PMID 8494055. 
  • Chroneos ZC, Abdolrasulnia R, Whitsett JA, Rice WR, Shepherd VL (1996). "Purification of a cell-surface receptor for surfactant protein A.". J. Biol. Chem. 271 (27): 16375–83. doi:10.1074/jbc.271.27.16375. PMID 8663107. 
  • Planer BC, Ning Y, Kumar SA, Ballard PL (1997). "Transcriptional regulation of surfactant proteins SP-A and SP-B by phorbol ester.". Biochim. Biophys. Acta 1353 (2): 171–9. doi:10.1016/S0167-4781(97)00070-5. PMID 9294011.