In ascidians the test is composed largely of cellulose (historically termed "tunicine"). From 1845 (when this was discovered by Schmidt) until 1958 (when cellulose fibres were found in mammalian connective tissue), ascidians were believed to be the only animals that synthesised cellulose.
- Endean, The Test of the Ascidian, Phallusia mammillata, Quarterly Journal of Microscopical Science, Vol. 102, part 1, pp. 107-117, 1961.
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