U937 cell

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U937 cells are a model cell line used in biomedical research.[1] They were isolated from the histiocytic lymphoma of a 37-year-old male patient and are used to study the behaviour and differentiation of monocytes. U937 cells mature and differentiate in response to a number of soluble stimuli, adopting the morphology and characteristics of mature macrophages.

U937 cells are of the myeloid lineage and so secrete a large number of cytokines and chemokines either constitutively (e.g. IL-1 and GM-CSF) or in response to soluble stimuli. TNFα and recombinant GM-CSF independently promote IL-10 production in U937 cells.

[2] IFNγ increases the expression of chemokine receptors (CCR1, CCR3 and CCR5) as well as that of nuclear receptors for melatonin.[3] IFNγ treatment enhances thrombin-induced IL-8 production[4] and is required for the melatonin-induced production of IL-6.[5]

U937 can be grown in DMEM or 1640 medium plus 10% fetal bovine serum.

Atromentin induces apoptosis in human leukemia U937 cells.[6]

The HLA alleles present in U937 cells are HLA-A*03:01, A*31:01, B*18:01, B*51:01, Cw*01:02 and Cw*07:02.[7]


  1. ^ "U937 - Cytokines and Cells Online Pathfinder Encyclopaedia". 
  2. ^ Lehmann MH (June 1998). "Recombinant human granulocyte-macrophage colony-stimulating factor triggers interleukin-10 expression in the monocytic cell line U937". Mol. Immunol. 35 (8): 479–485. doi:10.1016/S0161-5890(98)00043-1. PMID 9798652. 
  3. ^ Zella D, Barabitskaja O, Burns JM, et al. (June 1998). "Interferon-gamma increases expression of chemokine receptors CCR1, CCR3, and CCR5, but not CXCR4 in monocytoid U937 cells". Blood 91 (12): 4444–50. PMID 9616137. 
  4. ^ Suk K, Cha S (April 1999). "Thrombin-induced interleukin-8 production and its regulation by interferon-gamma and prostaglandin E2 in human monocytic U937 cells". Immunol. Lett. 67 (3): 223–27. doi:10.1016/S0165-2478(99)00015-2. PMID 10369130. 
  5. ^ Guerrero JM, Pozo D, García-Mauriño S, et al. (May–August 2002). "Nuclear receptors are involved in the enhanced IL-6 production by melatonin in U937 cells". Biol. Sign. Recep. 9 (3-4): 197–202. PMID 10899704. 
  6. ^ Atromentin-Induced Apoptosis in Human Leukemia U937 Cells. Kim Jin Hee and Choong Hwan Lee, Journal of microbiology and biotechnology, 2009, vol. 19, no9, pages 946-950, INIST:21945937
  7. ^ Gebreselassie D (November 2006). "Sampling of major histocompatibility complex class I-associated peptidome suggests relatively looser global association of HLA-B*5101 with peptides.". Hum. Immunol. 67 (11): 894–906. doi:10.1016/j.humimm.2006.08.294. PMC 2269730. PMID 17145369.