U937 cells are a model cell line used in biomedical research. They were isolated from the histiocytic lymphoma of a 37-year-old male patient and are used to study the behaviour and differentiation of monocytes. U937 cells mature and differentiate in response to a number of soluble stimuli, adopting the morphology and characteristics of mature macrophages.
U937 cells are of the myeloid lineage and so secrete a large number of cytokines and chemokines either constitutively (e.g. IL-1 and GM-CSF) or in response to soluble stimuli. TNFα and recombinant GM-CSF independently promote IL-10 production in U937 cells.
 IFNγ increases the expression of chemokine receptors (CCR1, CCR3 and CCR5) as well as that of nuclear receptors for melatonin. IFNγ treatment enhances thrombin-induced IL-8 production and is required for the melatonin-induced production of IL-6.
U937 can be grown in DMEM or 1640 medium plus 10% fetal bovine serum.
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