Pseudopeptidoglycan
![](http://upload.wikimedia.org/wikipedia/commons/thumb/b/b0/Pseudopeptidoglycan.png/220px-Pseudopeptidoglycan.png)
Pseudopeptidoglycan (also known as pseudomurein;[2] PPG hereafter) is a major cell wall component of some Archaea that differs from bacterial peptidoglycan in chemical structure, but resembles bacterial peptidoglycan in function and physical structure. Pseudopeptidoglycan, in general, is only present in a few methanogenic archaea. The basic components are N-acetylglucosamine and N-acetyltalosaminuronic acid (bacterial peptidoglycan containing N-acetylmuramic acid instead), which are linked by β-1,3-glycosidic bonds.[3]
Lysozyme, a host defense mechanism present in human secretions (e.g. saliva and tears) breaks β-1,4-glycosidic bonds to degrade peptidoglycan. However, because pseudopeptidoglycan has β-1,3-glycosidic bonds, lysozyme is ineffective. It was thought from these large differences in cell wall chemistry that archaeal cell walls and bacterial cell walls have not evolved from a common ancestor but are only the result of a convergent evolution,[4] but recent structural work has revealed deeper homology.[1]
No archaeal enzymes are known that cleave the β-1,3-glycosidic bonds in pseudopeptidoglycan, but it can be degraded by pseudomurein endoisopeptidase encoded by two prophages.[5] The pseudomurein endoisopeptidases function by cleaving the peptide links between adjacent pseudopeptidoglycan strands.
Structure
Pseudopeptidoglycan is composed of two sugars, N-acetylglucosamine and N-acetyltalosaminuronic acid. These sugars are made of different amino acids, and the peptide cross-links within pseudopeptidoglycan are formed with different amino acids. The peptide bond is formed between the lysine of a N-acetyltalosaminuronic acid and a glutamine of a parallel N-acetyltalosaminuronic acid.[6] Pseudopeptidoglycan, like peptidoglycan in bacteria, forms a mesh-like layer outside of the plasma membrane of the archaea.
Function
Only a few methanogenic archaea have cell walls composed of pseudopeptidoglycan. This component functions much like peptidoglycan in a bacterial cell.[7] Pseudopeptidoglycan is used by the archaeal cell to determine its shape and provide structure to the cell. It is also used to protect the cell from undesired molecules or anything harmful in its environment.
Biosynthesis
PPG is produced by enzymes of two gene clusters. Recent work on the peptide ligases show, surprisingly, a common origin with murein synthesis. The pathway is now known to include the orthologous-to-bacteria CarB, MurC/D (peptide ligase), MurG, MraY, UppP, UppS, and flippase presumably performing an analogous function, and two novel but conserved transmembrane proteins. GlmM and GlmU, which produce UDP-GlcNAc in bacteria, are also present with phosphoglucomutase (PGM). Half of the species also have MurT and GatD, known to perform cell wall modifications in bacteria. No orthologous cross-linking enzymes have been identified. Notably, "formation of the disaccharide moiety of the glycopeptide monomer occurs before the transfer to membrane protein by MraY", as opposed to after in bacteria. Further work would be needed to connect these information into a coherent pathway.[1]
Effects of different bacterial medicines on pseudopeptidoglycan
Lysozyme
Lysozyme is a natural defense mechanism in humans that has the ability to break down peptidoglycan in bacterial cells. It degrades the peptidoglycan by targeting the β-1,4-glycosidic bonds that connect the alternating amino sugars in which it is composed of.[8] This degradation of the glycosidic bonds within peptidoglycan cause the sugars to separate and inhibit the structural integrity of the peptidoglycan and the bacteria.
Pseudopeptidoglycan, however, is composed of a different acidic amino sugar, which is N-acetyltalosaminuronic acid. This difference is the reason that it has β-1,3-glycosidic bonds (as opposed to the β-1,4-glycosidic bonds in bacteria).[3] Lysozymes targets the linkage in peptidoglycan, and without that, becomes ineffective against pseudopeptidoglycan.
Penicillin
Penicillin is a group of antibiotics that have been effective against many bacterial infections. It attacks bacteria by targeting and inhibiting the transpeptidase that catalyzes the cross-linking of the amino sugars in peptidoglycan.[9] However, pseudopeptidoglycan contains different amino sugars, and therefore, a different catalysis enzyme is used. The different amino acids cause antibiotics, that target cell walls like penicillin, to be ineffective against pseudopeptidoglycan.[6]
Taxonomic distribution
PPG is found in the archaeal orders of Methanobacteriales and Methanopyrales.[1] Some genera under these orders are:
See also
References
- ^ a b c d Subedi, Bishwa P; Martin, William F; Carbone, Vincenzo; Duin, Eduardus C; Cronin, Bryan; Sauter, Julia; Schofield, Linley R; Sutherland-Smith, Andrew J; Ronimus, Ron S (7 September 2021). "Archaeal pseudomurein and bacterial murein cell wall biosynthesis share a common evolutionary ancestry". FEMS Microbes. 2: xtab012. doi:10.1093/femsmc/xtab012. PMC 10117817. PMID 37334239.
- ^ White, David. (1995) The Physiology and Biochemistry of Prokaryotes, pages 6, 12-21. (Oxford: Oxford University Press). ISBN 0-19-508439-X.
- ^ a b Albers, Sonja; Eichler, Jerry; Aebi, Markus (2015), Varki, Ajit; Cummings, Richard D.; Esko, Jeffrey D.; Stanley, Pamela (eds.), "Archaea", Essentials of Glycobiology (3rd ed.), Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press, doi:10.1101/glycobiology.3e.022 (inactive 31 January 2024), PMID 28876866, retrieved 2021-04-19
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: CS1 maint: DOI inactive as of January 2024 (link) - ^ Visweswaran, Ganesh Ram R.; Dijkstra, Bauke W.; Kok, Jan (2011). "Murein and pseudomurein cell wall binding domains of bacteria and archaea—a comparative view". Applied Microbiology and Biotechnology. 92 (5): 921–928. doi:10.1007/s00253-011-3637-0. ISSN 0175-7598. PMC 3210951. PMID 22012341.
- ^ Visweswaran, Ganesh Ram R.; Dijkstra, Bauke W.; Kok, Jan (2010). "Two Major Archaeal Pseudomurein Endoisopeptidases: PeiW and PeiP". Archaea. 2010: 480492. doi:10.1155/2010/480492. PMC 2989375. PMID 21113291.
- ^ a b Slonczewski, Joan, Watkins, John, Foster.; Slonczewski, Joan (2009). Microbiology: An Evolving Science.
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: CS1 maint: multiple names: authors list (link)[better source needed] - ^ "Peptidoglycan vs Pseudopeptidoglycan | Easy Biology Class". www.easybiologyclass.com. 2017-05-19. Retrieved 2021-05-06.
- ^ Primo, Emiliano D.; Otero, Lisandro H.; Ruiz, Francisco; Klinke, Sebastián; Giordano, Walter (2018). "The disruptive effect of lysozyme on the bacterial cell wall explored by an in-silico structural outlook". Biochemistry and Molecular Biology Education. 46 (1): 83–90. doi:10.1002/bmb.21092. ISSN 1539-3429. PMID 29131507.
- ^ Yocum, R. R.; Rasmussen, J. R.; Strominger, J. L. (1980-05-10). "The mechanism of action of penicillin. Penicillin acylates the active site of Bacillus stearothermophilus D-alanine carboxypeptidase". The Journal of Biological Chemistry. 255 (9): 3977–3986. doi:10.1016/S0021-9258(19)85621-1. ISSN 0021-9258. PMID 7372662.
Further reading
- Pohlschroder, Mechthild; Pfeiffer, Friedhelm; Schulze, Stefan; Halim, Mohd Farid Abdul (1 September 2018). "Archaeal cell surface biogenesis". FEMS Microbiology Reviews. 42 (5): 694–717. doi:10.1093/femsre/fuy027. PMC 6098224. PMID 29912330.