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RNF113A

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This is an old revision of this page, as edited by Alicia Florrick (talk | contribs) at 12:28, 24 August 2015 (Disambiguated: conservation → conservation (ethic)). The present address (URL) is a permanent link to this revision, which may differ significantly from the current revision.

Ring Finger Protein 113A is a protein that in humans is encoded by the RNF113A gene. It is found in humans on the X Chromosome. RNF113A contains two highly conserved domains, the RING (Really Interesting New Gene) finger domain and Zinc finger domain.[1] RING finger domains have been associated with some tumor suppressors and cytokine receptor-associated molecules. These domains also act in DNA repair and mediating protein-protein interactions.[1][2] Aliases of RNF113A across taxa include RNF113, CWC24, and ZNF183.

Gene

The gene is found on the human X Chromosome and reverse strand. The specific locus in humans is Xq24.[1] RNF113A contains 1312 nucleotides.

The red bar in white band q24 represents the location of the gene RFN113A on the human X chromosome.

Gene Structure

An upstream in-frame stop codon is found within the 5' UTR. RNF113A is an intronless gene with one isoform in humans.[1]

Protein

RNF113A translates a human protein 343 amino acids long and molecular weight of 38.8 kiloDaltons.[3] The protein is found ubiquitously in the human body.[4][5]

Yeast Two Hybrid Screens link RNF113A with other proteins. Most of these proteins are currently known to function in the human Spliceosome.[6] Some of these associations are within the U4, U5, and U6 snRNPs much the same as within yeast models.[7]

Protein Structure

RNF113A also contains one acetylation and four phosphorylation sites.[1] The protein has both an acetylation and four phosphorylation sites which have been confirmed experimentally.[8][9][10][11][12] Additional phosphorylation sites and one glycosylation site are also predicted.[13] The N terminus or 3' end of the gene contains the conserved RING and Zinc finger domains. The RING finger domain contains a cross-brace motif consisting of 6 Cystines and 1 Histidine.The Zinc finger is formed by 3 Cystines and 1 Histidine[14][15][16][17][18][19] Typically, RING finger domains are located near the C terminus or 5' end of the protein rather than the N terminus making RNF113A unique. RING finger proteins also usually have multiple types of domains outside of the Zinc finger family.[5]

The C-terminus end of the early isolated paralog RNF113B, formerly known as Zing Finger Protein 183-like 1, contains the RING domain in yellow to the C-terminus. The known alpha-helices and beta-sheets are visible. The two grey spheres represent zinc atoms.

Secondary structure of the RING domain has been confirmed for the paralog, RNF113B. Two Beta sheets and one Alpha helix are present within the domain.[20] A second Alpha helix is present on the 5' side of the RING domain.

Function

Human

The RNF113A protein was identified as a phosphoprotein in a human prostate cancer cell line but the function was not tested.[21] Online Mendelian Inheritance in Man (OMIM) does not recognize any diseases linked RNF113A. One case study has been reported in which the normally autosomal recessive trichothiodystrophy is under X-linked recessive inheritance control. RNF113A contains a mutation changing a cytosine to a tyrosine that creates a nonsense mutation. Mothers are the carriers for the disease and display only slightly altered phenotypes that were never linked to the mutation. myelodysplastic syndrome and 5q-syndrome have also been linked to an upregulation of ZNF183, an alias of RNF113A.[22] It appears RNF113A may allow for a more stable activated spliceosome and post-catalytic spliceosome.[23][24]

Yeast

The yeast ortholog Cwc24p is predicted to have a spliceosome function.[25] The protien acts in a complex with Cef1p to process pre-rRNA. The splicing is dependent on the Zinc finger and RING finger domains.[26]

Drosophila

The ortholog in fruit flies has been suggested to act as a spliceosome. Based on the observed phenotype of incomplete neuroblast differentiation, the ortholog is hypothesized to be involved in splicing namely within the central nervous system.[27] Additional research conclude a cytosine to tyrosine nonsense mutation such as that of trichothiodystrophy discussed above has resulted in abnormal development in which tissues of the ectoderm germ layer are affected.[28]

Nematodes

The Caenorhabditis elegans Tag-331 ortholog has been linked to larval arrest and legality when a knock-out is created[29] The RNF-113 ortholog has been predicted to function as an ubiquitin ligase that is involved in DNA repair of inter-strand crosslinks[30]

Paralog

RNF113B is the primate-specific retrogene of RNF113A.[31] The gene is a rare example of intron gain into a gene. In humans, RNF113B is found on Chromosome 13.[32] RNF113B mRNA transcript contains a upstream in-frame stop codon. The protein has both a RING finger domain (really interesting new gene) and a zinc finger motif.[33]

RNF113B currently is not associated with any human diseases according to the Online Mendelian Inheritance in Man (OMIM) database. Preliminary research has suggested the gene to be linked to development and differentiation.[34] RNF113B has also been predicted to be a part of the ubiquitin ligase family and involved with DNA repair mechanisms after treatment with cisplatin, a chemotherapy drug that induces DNA inter-strand crosslinks.[31][35] Further research indicates RNF113B is transcribed in a wide assortment of tissues. The transcripts can be spliced or unspliced and this action is specific to the tissue of expression. However, the mechanisms and functions of this gene specially in these tissues are still unknown.

Homology

Orthologs have been found in mammals, birds, reptiles, amphibians, fish, and invertebrates. Distant orthologs have been recognized in fungi, yeast, and plants. The zinc finger domain and RING finger domain are the regions of highest conservation. The upstream region displays the most conservation in mammals.

Scientific name Common name E value Query cover Identity Accession Protein length Taxa Divergence (myr)
Macaca mulatta Rhesus monkey 0 1.00 0.98 NP_001185630.1 344 Mammal 26.8
Equus caballus Horse 0 1.00 0.93 XP_001491864.1 344 Mammal 96.2
Chrymsemys picta bellii Western painted turtle 0 0.94 0.80 XP_005309675.1 323 Reptile 322.4
Gallus gallus Chicken 9E-177 0.93 0.77 NP_001004396.1 328 Bird 322.4
Xenopus laevis African clawed frog 5E-157 0.90 0.71 AAR97523.1 319 Amphibian 359.1
Danio rerio Zebrafish 5E-160 0.98 0.71 NP_001004536.1 321 Fish 436.8
Echinococcus multilocularis Flatworm 2E-100 0.90 0.5 CDI98689.1 389 Flatworm 625
Apis florea Little honeybee 2E-130 0.88 0.62 XP_003695009.1 325 Insect 725.5
Ciona intestinalis Vase Tunicate 2E-127 0.92 0.61 NP_001027830.1 325 Tunicate 763.5
Saccharomyces cerevisiae Fungus 4E-40 0.59 0.44 NP_013427.1 259 Yeast 1211
Amorella trichopoda Shrub 4E-62 0.92 0.40 XP_006842511.1 322 Plant 1375

The table above displays the results of an NCBI Blast from 2015 with selected taxa from main branches of vertebrates and invertebrates. This is not a complete list.

References

  1. ^ a b c d e "Homo sapiens ring finger protein 113A (RNF113A), mRNA". NCBI Nucleotide. Retrieved 30 April 2015.
  2. ^ "RNF113A ring finger protein 113A [ Homo sapiens (human) ]". NCBI Gene. Retrieved 30 April 2015.
  3. ^ "RING finger protein 113A [Homo sapiens]". NCBI Protein. Retrieved 2 May 2015.
  4. ^ Identification of a new member (ZNF183) of the Ring finger gene family in Xq24-25
  5. ^ a b Frattini, Annalisa; Faranda, Sara; Bagnasco, Luca; Patrosso, Cristina; Nulli, Paola; Zucchi, Ileana; Vezzoni, Paolo (June 1997). "Identification of a new member (ZNF183) of the Ring finger gene family in Xq24-25". Gene. 192 (2): 291–298. doi:10.1016/S0378-1119(97)00108-X.
  6. ^ Hegele, Anna; Kamburov, Atanas; Grossmann, Arndt; Sourlis, Chrysovalantis; Wowro, Sylvia; Weimann, Mareike; Will, Cindy L.; Pena, Vlad; Lührmann, Reinhard; Stelzl, Ulrich (February 2012). "Dynamic Protein-Protein Interaction Wiring of the Human Spliceosome". Molecular Cell. 45 (4): 567–580. doi:10.1016/j.molcel.2011.12.034.
  7. ^ Coltri, Patricia P.; Oliveira, Carla C.; Maas, Stefan (24 September 2012). "Cwc24p Is a General Saccharomyces cerevisiae Splicing Factor Required for the Stable U2 snRNP Binding to Primary Transcripts". PLoS ONE. 7 (9): e45678. doi:10.1371/journal.pone.0045678.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  8. ^ Mayya, V.; Lundgren, D. H.; Hwang, S.-I.; Rezaul, K.; Wu, L.; Eng, J. K.; Rodionov, V.; Han, D. K. (18 August 2009). "Quantitative Phosphoproteomic Analysis of T Cell Receptor Signaling Reveals System-Wide Modulation of Protein-Protein Interactions". Science Signaling. 2 (84): ra46–ra46. doi:10.1126/scisignal.2000007.
  9. ^ Dephoure, N.; Zhou, C.; Villen, J.; Beausoleil, S. A.; Bakalarski, C. E.; Elledge, S. J.; Gygi, S. P. (31 July 2008). "A quantitative atlas of mitotic phosphorylation". Proceedings of the National Academy of Sciences. 105 (31): 10762–10767. doi:10.1073/pnas.0805139105.
  10. ^ Rigbolt, K. T. G.; Prokhorova, T. A.; Akimov, V.; Henningsen, J.; Johansen, P. T.; Kratchmarova, I.; Kassem, M.; Mann, M.; Olsen, J. V.; Blagoev, B. (15 March 2011). "System-Wide Temporal Characterization of the Proteome and Phosphoproteome of Human Embryonic Stem Cell Differentiation". Science Signaling. 4 (164): rs3–rs3. doi:10.1126/scisignal.2001570.
  11. ^ Olsen, J. V.; Vermeulen, M.; Santamaria, A.; Kumar, C.; Miller, M. L.; Jensen, L. J.; Gnad, F.; Cox, J.; Jensen, T. S.; Nigg, E. A.; Brunak, S.; Mann, M. (12 January 2010). "Quantitative Phosphoproteomics Reveals Widespread Full Phosphorylation Site Occupancy During Mitosis". Science Signaling. 3 (104): ra3–ra3. doi:10.1126/scisignal.2000475.
  12. ^ Gauci, Sharon; Helbig, Andreas O.; Slijper, Monique; Krijgsveld, Jeroen; Heck, Albert J. R.; Mohammed, Shabaz (June 2009). "Lys-N and Trypsin Cover Complementary Parts of the Phosphoproteome in a Refined SCX-Based Approach". Analytical Chemistry. 81 (11): 4493–4501. doi:10.1021/ac9004309.
  13. ^ "NetPhos 2.0". ExPasy. Retrieved 2 May 2015.
  14. ^ NCBI Protein NP_008909.1 http://www.ncbi.nlm.nih.gov/protein/NP_008909.1
  15. ^ "zinc finger protein 183 (RING finger, C3HC4 type) [Homo sapiens]". NCBI Protein. Retrieved 30 April 2015.
  16. ^ "ring finger protein 113A [Homo sapiens]". NCBI Protein. Retrieved 30 April 2015.
  17. ^ "ZNF183 [Homo sapiens]". NCBI Protein. Retrieved 30 April 2015.
  18. ^ "Ring finger protein 113A [Homo sapiens]". NCBI Protien. Retrieved 30 April 2015.
  19. ^ "Ring finger protein 113A [Homo sapiens]". NCBI Protein. Retrieved 30 April 2015.
  20. ^ "MMDB Protein Structure Summary". NCBI Structure. Retrieved 2 May 2015.
  21. ^ Giorgianni, Francesco; Zhao, Yingxin; Desiderio, Dominic M.; Beranova-Giorgianni, Sarka (June 2007). "Toward a global characterization of the phosphoproteome in prostate cancer cells: Identification of phosphoproteins in the LNCaP cell line". ELECTROPHORESIS. 28 (12): 2027–2034. doi:10.1002/elps.200600782.
  22. ^ Pellagatti, Andrea; Esoof, Noor; Watkins, Fiona; Langford, Cordelia F.; Vetrie, David; Campbell, Lisa J.; Fidler, Carrie; Cavenagh, James D.; Eagleton, Helen; Gordon, Peter; Woodcock, Barrie; Pushkaran, Beena; Kwan, Mark; Wainscoat, James S.; Boultwood, Jacqueline (June 2004). "Gene expression profiling in the myelodysplastic syndromes using cDNA microarray technology". British Journal of Haematology. 125 (5): 576–583. doi:10.1111/j.1365-2141.2004.04958.x.
  23. ^ Ilagan, J. O.; Chalkley, R. J.; Burlingame, A. L.; Jurica, M. S. (23 January 2013). "Rearrangements within human spliceosomes captured after exon ligation". RNA. 19 (3): 400–412. doi:10.1261/rna.034223.112.
  24. ^ Bessonov, S.; Anokhina, M.; Krasauskas, A.; Golas, M. M.; Sander, B.; Will, C. L.; Urlaub, H.; Stark, H.; Luhrmann, R. (27 October 2010). "Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis". RNA. 16 (12): 2384–2403. doi:10.1261/rna.2456210.
  25. ^ Fabrizio, Patrizia; Dannenberg, Julia; Dube, Prakash; Kastner, Berthold; Stark, Holger; Urlaub, Henning; Lührmann, Reinhard (November 2009). "The Evolutionarily Conserved Core Design of the Catalytic Activation Step of the Yeast Spliceosome". Molecular Cell. 36 (4): 593–608. doi:10.1016/j.molcel.2009.09.040.
  26. ^ Goldfeder, M. B.; Oliveira, C. C. (1 November 2007). "Cwc24p, a Novel Saccharomyces cerevisiae Nuclear Ring Finger Protein, Affects Pre-snoRNA U3 Splicing". Journal of Biological Chemistry. 283 (5): 2644–2653. doi:10.1074/jbc.M707885200.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  27. ^ Carney, T. D.; Struck, A. J.; Doe, C. Q. (11 September 2013). "midlife crisis encodes a conserved zinc-finger protein required to maintain neuronal differentiation in Drosophila". Development. 140 (20): 4155–4164. doi:10.1242/dev.093781.
  28. ^ Corbett, M. A.; Dudding-Byth, T.; Crock, P. A.; Botta, E.; Christie, L. M.; Nardo, T.; Caligiuri, G.; Hobson, L.; Boyle, J.; Mansour, A.; Friend, K. L.; Crawford, J.; Jackson, G.; Vandeleur, L.; Hackett, A.; Tarpey, P.; Stratton, M. R.; Turner, G.; Gecz, J.; Field, M. (22 January 2015). "A novel X-linked trichothiodystrophy associated with a nonsense mutation in RNF113A". Journal of Medical Genetics. 52 (4): 269–274. doi:10.1136/jmedgenet-2014-102418.
  29. ^ Haerty, Wilfried; Artieri, Carlo; Khezri, Navid; Singh, Rama S; Gupta, Bhagwati P (2008). "Comparative analysis of function and interaction of transcription factors in nematodes: Extensive conservation of orthology coupled to rapid sequence evolution". BMC Genomics. 9 (1): 399. doi:10.1186/1471-2164-9-399.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  30. ^ Lee, Hyojin; Alpi, Arno F.; Park, Mi So; Rose, Ann; Koo, Hyeon-Sook; Leng, Fenfei (28 March 2013). "C. elegans Ring Finger Protein RNF-113 Is Involved in Interstrand DNA Crosslink Repair and Interacts with a RAD51C Homolog". PLoS ONE. 8 (3): e60071. doi:10.1371/journal.pone.0060071.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  31. ^ a b Szczesniak, M. W.; Ciomborowska, J.; Nowak, W.; Rogozin, I. B.; Makalowska, I. (1 October 2010). "Primate and Rodent Specific Intron Gains and the Origin of Retrogenes with Splice Variants". Molecular Biology and Evolution. 28 (1): 33–37. doi:10.1093/molbev/msq260.
  32. ^ "Homo sapiens ring finger protein 113B (RNF113B), mRNA". NCBI Nucleotide. Retrieved 2 May 2015.
  33. ^ "RING finger protein 113B [Homo sapiens]". NCBI Protein. Retrieved 2 May 2015.
  34. ^ Czugala, Marta; Karolak, Justyna A; Nowak, Dorota M; Polakowski, Piotr; Pitarque, Jose; Molinari, Andrea; Rydzanicz, Malgorzata; Bejjani, Bassem A; Yue, Beatrice Y J T; Szaflik, Jacek P; Gajecka, Marzena (2 November 2011). "Novel mutation and three other sequence variants segregating with phenotype at keratoconus 13q32 susceptibility locus". European Journal of Human Genetics. 20 (4): 389–397. doi:10.1038/ejhg.2011.203.
  35. ^ Carroll, Eilis. "Investigation into ubiquitin signalling in response to cisplatin". Discovery Research Portal. University of Dundee. Retrieved 2 May 2015.
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