Oinopota Kirby & Spence, 1815
- 1 Etymology
- 2 Morphology
- 3 Lifecycle and ecology
- 4 Systematics
- 5 Drosophila species genome project
- 6 See also
- 7 References
- 8 External links
Drosophila species are small flies, typically pale yellow to reddish brown to black, with red eyes. Many species, including the noted Hawaiian picture-wings, have distinct black patterns on the wings. The plumose (feathery) arista, bristling of the head and thorax, and wing venation are characters used to diagnose the family. Most are small, about 2–4 mm long, but some, especially many of the Hawaiian species, are larger than a house fly.
Lifecycle and ecology
Drosophila species are found all around the world, with more species in the tropical regions. Drosophila made their way to the Hawaiian Islands and radiated into over 800 species. They can be found in deserts, tropical rainforest, cities, swamps, and alpine zones. Some northern species hibernate. Most species breed in various kinds of decaying plant and fungal material, including fruit, bark, slime fluxes, flowers, and mushrooms. The larvae of at least one species, D. suzukii, can also feed in fresh fruit and can sometimes be a pest. A few species have switched to being parasites or predators. Many species can be attracted to baits of fermented bananas or mushrooms, but others are not attracted to any kind of baits. Males may congregate at patches of suitable breeding substrate to compete for the females, or form leks, conducting courtship in an area separate from breeding sites.
Several Drosophila species, including D. melanogaster, D. immigrans, and D. simulans, are closely associated with humans, and are often referred to as domestic species. These and other species (D. subobscura, Zaprionus indianus) have been accidentally introduced around the world by human activities such as fruit transports.
Males of this genus are known to have the longest sperm cells of any studied organism on Earth, including one species, Drosophila bifurca, that has sperm cells that are 58 mm (2.3 in) long. The cells are mostly tail, and are delivered to the females in tangled coils. The other members of the genus Drosophila also make relatively few giant sperm cells, with that of D. bifurca being the longest. D. melanogaster sperm cells are a more modest 1.8 mm long, although this is still about 35 times longer than a human sperm. Several species in the D. melanogaster species group are known to mate by traumatic insemination.
Drosophila species vary widely in their reproductive capacity. Those such as D. melanogaster that breed in large, relatively rare resources have ovaries that mature 10–20 eggs at a time, so that they can be laid together on one site. Others that breed in more-abundant but less nutritious substrates, such as leaves, may only lay one egg per day. The eggs have one or more respiratory filaments near the anterior end; the tips of these extend above the surface and allow oxygen to reach the embryo. Larvae feed not on the vegetable matter itself, but on the yeasts and microorganisms present on the decaying breeding substrate. Development time varies widely between species (between 7 and more than 60 days) and depends on the environmental factors such as temperature, breeding substrate, and crowding. Fruit flies lay eggs in response to environmental cycles. Eggs laid at a time (e.g., night) during which likelihood of survival is greater than in eggs laid at other times (e.g., day) yield more larvae than eggs that were laid at those times. Ceterus paribus, the habit of laying eggs at this 'advantageous' time would yield more surviving offspring, and more grandchildren, than the habit of laying eggs during other times. This differential reproductive success would cause D. melanogaster to adapt to environmental cycles, because this behavior has a major reproductive advantage.
Their median lifespan is 35–45 days.
The following section is based on the following Drosophila species: Drosophila simulans, and Drosophila melanogaster. Courtship behavior of male Drosophila is an attractive behaviour. Females respond via their perception of the behavior portrayed by the male. Male and female Drosophila use innate complex behaviors use a variety of sensory cues to initiate and assess courtship readiness of a potential mate. The cues include the following behaviours: positioning, pheromone excretion, following females, making tapping sounds with legs, singing, wing spreading, creating wing vibrations, genitalia licking, bending the stomach, attempt to copulate, and the copulatory act itself. The songs of Drosophila melanogaster and Drosophila simulans have been studied extensively. These luring songs are sinusoidal in nature and varies within and between species. The courtship behavior of Drosophila melanogaster has also been assessed for sex-related genes, which have been implicated in courtship behavior in both the male and female. Recent experiments explore the role of fruitless (fru) and doublesex (dsx), a group of sex-behaviour linked genes. This research is currently being explored.
The following section is based on the following Drosophila species: Drosophila serrata, Drosophila pseudoobscura, Drosophila melanogaster, and Drosophila neotestacea. Polyandry is a prominent mating system among Drosophila. Females mating with multiple sex partners has been a beneficial mating strategy for Drosophila. The benefits include both pre and post copulatory mating. Pre-copulatory strategies are the behaviours associated with mate choice and the genetic contributions, such as production of gametes, that are exhibited by both male and female Drosophila regarding mate choice. Post copulatory strategies include sperm competition, mating frequency, and sex-ratio meiotic drive. These lists are not inclusive. Polyandry among the Drosophila pseudoobscura in North America vary in their number of mating partners. There is a connection between the number of time females choose to mate and chromosomal variants of the third chromosome. It is believed that the presence of the inverted polymorphism is why re-mating by females occurs. The stability of these polymorphisms may be related to the sex-ratio meiotic drive.
The following section is based on the following Drosophila species: Drosophila melanogaster, Drosophila simulans, and Drosophila mauritiana. Sperm competition is a process that polyandrous Drosophila females use to increase the fitness of their offspring. The female Drosophila has two sperm storage organs that allows her to choose the sperm that will be used to inseminate her eggs. Females have little control when it comes to cryptic female choice. Female Drosophila through cryptic choice, one of several post-copulatory mechanisms, which allows for the detection and expelling of sperm that reduces inbreeding possibilities. Manier et al. 2013 has categorized the post copulatory sexual selection of Drosophila melanogaster, Drosophila simulans, and Drosophila mauritiana into the following three stages: insemination, sperm storage, and fertilizable sperm. Among the preceding species there are variations at each stage that play a role in the natural selection process.
D. melanogaster is a popular experimental animal because it is easily cultured en masse out of the wild, has a short generation time, and mutant animals are readily obtainable. In 1906, Thomas Hunt Morgan began his work on D. melanogaster and reported his first finding of a white eyed mutant in 1910 to the academic community. He was in search of a model organism to study genetic heredity and required a species that could randomly acquire genetic mutation that would visibly manifest as morphological changes in the adult animal. His work on Drosophila earned him the 1933 Nobel Prize in Medicine for identifying chromosomes as the vector of inheritance for genes. This and other Drosophila species are widely used in studies of genetics, embryogenesis, chronobiology, speciation, neurobiology, and other areas.
However, some species of Drosophila are difficult to culture in the laboratory, often because they breed on a single specific host in the wild. For some, it can be done with particular recipes for rearing media, or by introducing chemicals such as sterols that are found in the natural host; for others, it is (so far) impossible. In some cases, the larvae can develop on normal Drosophila lab medium, but the female will not lay eggs; for these it is often simply a matter of putting in a small piece of the natural host to receive the eggs. The Drosophila Species Stock Center located at Cornell University in Ithaca, New York, maintains cultures of hundreds of species for researchers.
Like other metazoans, Drosophila is associated with various bacteria in its gut. The fly gut microbiota or microbiome seems to have a central influence on Drosophila fitness and life history characteristics. The microbiota in the gut of Drosophila represents an active current research field.
Drosophila species also harbour vertically transmitted endosymbionts such as Wolbachia and Spiroplasma. These endosymbionts can act as reproductive manipulators, such as cytoplasmic incompatibility induced by Wolbachia or male-killing induced by the D. melanogaster Spiroplasma poulsonii (named MSRO). The male-killing factor of the D. melanogaster MSRO strain was discovered in 2018, solving a decades-old mystery of the cause of male-killing. This represents the first bacterial factor that affects eukaryotic cells in a sex-specific fashion, and is the first mechanism identified for male-killing phenotypes. Alternatively, they may protect theirs hosts from infection. Drosophila Wolbachia can reduce viral loads upon infection, and is explored as a mechanism of controlling viral diseases (e.g. Dengue fever) by transferring these Wolbachia to disease-vector mosquitoes. The S. poulsonii strain of Drosophila neotestacea protects its host from parasitic wasps and nematodes using toxins that preferentially attack the parasites instead of the host.
Drosophila species are prey for many generalist predators such as robber flies. In Hawaii, the introduction of yellowjackets from the mainland United States has led to the decline of many of the large species. The larvae are preyed on by other fly larvae, staphylinid beetles, and ants.
The genus Drosophila as currently defined is paraphyletic (see below) and contains 1,450 described species, while the total number of species is estimated at thousands. The majority of the species are members of two subgenera: Drosophila (about 1,100 species) and Sophophora (including D. (S.) melanogaster; around 330 species). The Hawaiian species of Drosophila (estimated to be more than 500, with roughly 380 species described) are sometimes recognized as a separate genus or subgenus, Idiomyia, but this is not widely accepted. About 250 species are part of the genus Scaptomyza, which arose from the Hawaiian Drosophila and later recolonized continental areas.
- Liodrosophila Duda, 1922
- Mycodrosophila Oldenburg, 1914
- Samoaia Malloch, 1934
- Scaptomyza Hardy, 1849
- Zaprionus Coquillett, 1901
- Zygothrica Wiedemann, 1830
- Hirtodrosophila Duda, 1923 (position uncertain)
Several of the subgeneric and generic names are based on anagrams of Drosophila, including Dorsilopha, Lordiphosa, Siphlodora, Phloridosa, and Psilodorha.
Drosophila species genome project
Drosophila species are extensively used as model organisms in genetics (including population genetics), cell biology, biochemistry, and especially developmental biology. Therefore, extensive efforts are made to sequence drosphilid genomes. The genomes of these species have been fully sequenced:
- Drosophila (Sophophora) melanogaster
- Drosophila (Sophophora) simulans
- Drosophila (Sophophora) sechellia
- Drosophila (Sophophora) yakuba
- Drosophila (Sophophora) erecta
- Drosophila (Sophophora) ananassae
- Drosophila (Sophophora) pseudoobscura
- Drosophila (Sophophora) persimilis
- Drosophila (Sophophora) willistoni
- Drosophila (Drosophila) mojavensis
- Drosophila (Drosophila) virilis
- Drosophila (Drosophila) grimshawi
The data have been used for many purposes, including evolutionary genome comparisons. D. simulans and D. sechellia are sister species, and provide viable offspring when crossed, while D. melanogaster and D. simulans produce infertile hybrid offspring. The Drosophila genome is often compared with the genomes of more distantly related species such as the honeybee Apis mellifera or the mosquito Anopheles gambiae.
Curated data are available at FlyBase.
- Drosophila hybrid sterility
- Laboratory experiments of speciation
- List of Drosophila species
- Caenorhabditis 'Drosophilae' species supergroup, a group of species generally found on rotten fruits and transported by Drosophila flies
- Jones, Daniel (2003) , Peter Roach; James Hartmann; Jane Setter (eds.), English Pronouncing Dictionary, Cambridge: Cambridge University Press, ISBN 978-3-12-539683-8
- "Drosophila". Merriam-Webster Dictionary.
- Gerhard Bächli (1999–2006). "TaxoDros: the database on taxonomy of Drosophilidae".
- Program, U.S. Fish and Wildlife Service/Ecological Services. "Endangered Species | About Us | Featured Species: Relict Leopard Frog". www.fws.gov. Retrieved 2018-03-10.
- Mark Hoddle. "Spotted Wing Drosophila (Cherry Vinegar Fly) Drosophila suzukii". Center for Invasive Species Research. Retrieved July 29, 2010.
- C. R. Vilela (1999). "Is Zaprionus indianus Gupta, 1970 (Diptera, Drosophilidae) currently colonizing the Neotropical region?". Drosophila Information Service. 82: 37–39.
- Kim van der Linde; Gary J. Steck; Ken Hibbard; Jeffrey S. Birdsley; Linette M. Alonso; David Houle (2006). "First records of Zaprionus indianus (Diptera, Drosophilidae), a pest species on commercial fruits, from Panama and the United States of America". Florida Entomologist. 89 (3): 402–404. doi:10.1653/0015-4040(2006)89[402:FROZID]2.0.CO;2.
- S. Castrezana (2007). "New records of Zaprionus indianus Gupta, 1970 (Diptera, Drosophilidae) in North America and a key to identify some Zaprionus species deposited in the Drosophila Tucson Stock Center". Drosophila Information Service. 90: 34–36.
- Scott Pitnick; Greg S. Spicer; Therese A. Markow (1995). "How long is a giant sperm?". Nature. 375 (6527): 109. doi:10.1038/375109a0. PMID 7753164.
- Dominique Joly; Nathalie Luck; Béatrice Dejonghe (2007). "Adaptation to long sperm in Drosophila: correlated development of the sperm roller and sperm packaging". Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 310B (2): 167–178. doi:10.1002/jez.b.21167. PMID 17377954.
- Kamimura, Yoshitaka (2007-08-22). "Twin intromittent organs of Drosophila for traumatic insemination". Biol. Lett. 3 (4): 401–404. doi:10.1098/rsbl.2007.0192. PMC 2391172. PMID 17519186.
- Howlader, G., Sharma, K. V (2006). Circadian regulation of egg-laying behavior in fruit flies Drosophila melanogaster. The Journal of Insect Physiology, 52, 779–785.
- Broughton SJ, Piper MD, Ikeya T, Bass TM, Jacobson J, Driege Y, Martinez P, Hafen E, Withers DJ, Leevers SJ, Partridge L (2005). "Longer lifespan, altered metabolism, and stress resistance in Drosophila from ablation of cells making insulin-like ligands". Proceedings of the National Academy of Sciences of the United States of America. 102 (8): 3105–10. doi:10.1073/pnas.0405775102. PMC 549445. PMID 15708981.
- Pan, Y.; Robinett, C. C.; Baker, B. S. (2011). "Turning males on: Activation of male courtship behavior in Drosophila melanogaster". PLoS ONE. 6 (6): e21144. doi:10.1371/journal.pone.0021144. PMC 3120818. PMID 21731661.
- Cook, R. M. (1973). "Courtship Processing in Drosophila melanogaster. II. An Adaptation to Selection for Receptivity to Wingless Males". Animal Behaviour. 21 (2): 349–358. doi:10.1016/S0003-3472(73)80077-6.
- Crossley, S. A.; Bennet-Clark, H. C.; Evert, H. T. (1995). "Courtship song components affect male and female Drosophila differently". Animal Behaviour. 50 (3): 827–839. doi:10.1016/0003-3472(95)80142-1.
- Ejima A., and L.C. Griffith. 2007. Measurement of Courtship Behavior in Drosophila melanogaster. Medline
- Certel, S. J.; Savella, M. G.; Schlegel, D. C. F.; Kravitz, E. A. (2007). "Modulation of Drosophila male behavioral choice". Proceedings of the National Academy of Sciences. 104 (11): 4706–4711. doi:10.1073/pnas.0700328104. PMC 1810337. PMID 17360588.
- Frentiu, F. D.; Chenoweth, S. F. (2008). "Polandry and paternity skew in natural and experimental populations of Drosophila serrata". Molecular Ecology. 17 (6): 1589–1596. doi:10.1111/j.1365-294X.2008.03693.x. PMID 18266626.
- Puurtinen, M.; Fromhage, L. (2017). "Evolution of male and female choice in polyandrous systems". Proceedings of the Royal Society B: Biological Sciences. 284 (1851): 20162174. doi:10.1098/rspb.2016.2174. PMC 5378073. PMID 28330914.
- Herrera, P.; Taylor, M. L.; Skeats, A.; Price, T. A. R.; Wedell, N. (2014). "Can patterns of chromosome inversions in Drosophila pseudoobscura predict polyandry across a geographical cline?". Ecology and Evolution. 4 (15): 3072–3081. doi:10.1002/ece3.1165. PMC 4161180. PMID 25247064.
- Pinzone, C. A.; Dyer, K. A. (2013). "Association of polyandry and sex-ratio drive prevalence in natural populations of Drosophila neotestacea". Proceedings: Biological Sciences. 280 (1769): 20131397. doi:10.1098/rspb.2013.1397. PMC 3768301. PMID 24004936.
- Manier, M. K.; Belote, J. M.; Berben, K. S.; Lüpold, S.; Ala-Honkola, O.; Collins, W. F.; Pitnick, S. (2013). "Rapid Diversification Of Sperm Precedence Traits And Processes Among Three Sibling Drosophila Species". Evolution. 67 (8): 2348–2362. doi:10.1111/evo.12117. PMID 23888856.
- Clark, A. G.; Begun, D. J.; Prout, T. (1999). "Female X Male Interactions in Drosophila Sperm Competition". Science. 238 (5399): 217–220. doi:10.1126/science.283.5399.217. JSTOR 2897403.
- Mack, P. D.; Hammock, B. A.; Promislow, D. E. L. (2002). "Sperm competitive ability and genetic relatedness in Drosophila melanogaster: Similarity breeds contempt". Evolution. 56 (9): 1789–1795. doi:10.1111/j.0014-3820.2002.tb00192.x.
- Manier, M. K.; Lüpold, S.; Pitnick, S.; Starmer, W. T. (2013). "An Analytical Framework for Estimating Fertilization Bias and the Fertilization Set from Multiple Sperm-Storage Organs". The American Naturalist. 182 (4): 552–561. doi:10.1086/671782. PMID 24021407.
- Ala-Honkola, O.; Manier, M. K. (2016). "Multiple mechanisms of cryptic female choice act on intraspecific male variation in Drosophila simulans". Behavioral Ecology and Sociobiology. 70 (4): 519–532. doi:10.1007/s00265-016-2069-3.
- "The National Drosophila Species Stock Center | College of Agriculture and Life Science". blogs.cornell.edu. Retrieved August 6, 2018.
- Nik Papageorgiou. 2018. Mystery solved: The bacterial protein that kills male fruit flies. https://actu.epfl.ch/news/mystery-solved-the-bacterial-protein-that-kills--3/
- World Mosquito Program. http://www.eliminatedengue.com/our-research/wolbachia
- Haselkorn and Jaenike, 2016. https://onlinelibrary.wiley.com/doi/full/10.1111/mec.13261
- Hamilton et al. 2016. https://www.pnas.org/content/113/2/350
- Ballinger and Perlman. 2017. https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006431
- Therese A. Markow; Patrick M. O'Grady (2005). Drosophila: A guide to species identification and use. London: Elsevier. ISBN 978-0-12-473052-6.
- Colin Patterson (1999). Evolution. Cornell University Press. ISBN 978-0-8014-8594-7.
- Irina Brake; Gerhard Bächli (2008). Drosophilidae (Diptera). World Catalogue of Insects. pp. 1–412. ISBN 978-87-88757-88-0.
- Patrick O'Grady; Rob DeSalle (2008). "Out of Hawaii: the origin and biogeography of the genus Scaptomyza (Diptera: Drosophilidae)". Biology Letters. 4 (2): 195–199. doi:10.1098/rsbl.2007.0575. PMC 2429922. PMID 18296276.
- James Remsen; Patrick O'Grady (2002). "Phylogeny of Drosophilinae (Diptera: Drosophilidae), with comments on combined analysis and character support". Molecular Phylogenetics and Evolution. 24 (2): 249–264. doi:10.1016/S1055-7903(02)00226-9. PMID 12144760.
- "12 Drosophila Genomes Project". Lawrence Berkeley National Laboratory. Archived from the original on May 27, 2010. Retrieved July 29, 2010.
- "modEncode Comparative Genomics white paper" (PDF). ENCODE. Retrieved December 13, 2013.
- "i5k species nomination summary". Archived from the original on December 15, 2013. Retrieved December 13, 2013.
|Wikimedia Commons has media related to Drosophila.|
|Wikispecies has information related to Drosophila|
- Fly Base FlyBase is a comprehensive database for information on the genetics and molecular biology of Drosophila. It includes data from the Drosophila Genome Projects and data curated from the literature.
- Berkeley Drosophila Genome Project
- Annual Drosophila Research Conference
- AAA: Assembly, Alignment and Annotation of 12 Drosophila species
- UCSC Genome browser
- TaxoDros: The database on Taxonomy of Drosophilidae
- UC San Diego Drosophila Stock Center breeds hundreds of species and supplies them to researchers
- FlyMine is an integrated database of genomic, expression and protein data for Drosophila
- The Drosophila Virtual library is library of Drosophila on web
- Drosophila Melanogaster contains further information.
- C-CAMP Fly facility – In India microinjection service for the generation of transgenic lines, Screening Platforms, Drosophila strain development