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Calcein

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Calcein
Names
Preferred IUPAC name
2,2′,2′′,2′′′-[(3′,6′-Dihydroxy-3-oxo-3H-spiro[[2]benzofuran-1,9′-xanthene]-2′,7′-diyl)bis(methylenenitrilo)]tetraacetic acid
Other names
Fluorexon
Identifiers
3D model (JSmol)
ChEBI
ChEMBL
ChemSpider
ECHA InfoCard 100.014.507 Edit this at Wikidata
UNII
  • InChI=1S/C30H26N2O13/c33-21-7-23-19(5-15(21)9-31(11-25(35)36)12-26(37)38)30(18-4-2-1-3-17(18)29(43)45-30)20-6-16(22(34)8-24(20)44-23)10-32(13-27(39)40)14-28(41)42/h1-8,33-34H,9-14H2,(H,35,36)(H,37,38)(H,39,40)(H,41,42) checkY
    Key: DEGAKNSWVGKMLS-UHFFFAOYSA-N checkY
  • O=C(O5)c1ccccc1C(c3cc(CN(CC(O)=O)CC(O)=O)c(O)cc3O4)5c2c4cc(O)c(CN(CC(O)=O)CC(O)=O)c2
  • c1ccc2c(c1)C(=O)OC23c4cc(c(cc4Oc5c3cc(c(c5)O)CN(CC(=O)O)CC(=O)O)O)CN(CC(=O)O)CC(=O)O
Properties
C30H26N2O13
Molar mass 622.53 g/mol
Melting point Decomposes
Boiling point N/A
Slightly soluble
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
☒N verify (what is checkY☒N ?)

Calcein, also known as fluorexon, fluorescein complex, is a fluorescent dye with excitation and emission wavelengths of 495 and 515 nm, respectively, and has the appearance of orange crystals. Calcein self-quenches at concentrations above 70 mM and is commonly used as an indicator of lipid vesicle leakage.[1][2][3] It has also been traditionally used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

Applications

After Calcein-AM is taken up into the cell, it is converted by esterases into calcein (below). This is capable of complexing calcium ions, resulting in a green fluorescence. Since only living cells possess sufficient esterases, only live cells fluoresce green after excitation

The non-fluorescent acetomethoxy derivate of calcein (calcein AM, AM = acetoxymethyl) is used in biology as it can be transported through the cellular membrane into live cells, which makes it useful for testing of cell viability and for short-term labeling of cells. Alternatively, Fura-2 , Furaptra , Indo-1 and aequorin may be used. An acetomethoxy group obscures the part of the molecule that chelates Ca2+, Mg2+, Zn2+ and other ions. After transport into the cells, intracellular esterases remove the acetomethoxy group, the molecule gets trapped inside and gives out strong green fluorescence. As dead cells lack active esterases, only live cells are labeled[4] and counted by flow cytometry.

Human neonatal human dermal fibroblasts stained with calcein-AM, imaged with a monochromatic microscope, and pseudocolored.

Calcein is now rarely used as a Ca2+ or Mg2+ indicator because its fluorescence is directly sensitive to these ions only at strongly alkaline pH, and thus it is not particularly useful for measuring Ca2+ or Mg2+ in cells. Fluorescence of calcein is quenched strongly by Co2+, Ni2+ and Cu2+ and appreciably by Fe3+ and Mn2+ at physiological pH. This fluorescence quenching response can be exploited for detecting the opening of the mitochondrial permeability transition pore (mPTP) and for measuring cell volume changes.[5] Calcein is commonly used for cell tracing and in studies of endocytosis, cell migration, and gap junctions.[6]

The acetoxymethyl ester of calcein is also used to detect drug interactions with multidrug resistance proteins (ABC transporters ATP-binding cassette transporter genes) in intact cells as it is an excellent substrate of the multidrug resistance transporter 1 (MDR1) P-glycoprotein and the Multidrug Resistance-Associated Protein (MRP1).[7] The calcein AM assay can be used as a model for drug-drug interactions, for screening transporter substrates and/or inhibitors; and also to determine in vitro drug resistance of cells, including samples from patients.[8]

Calcein is also used for marking freshly hatched fish[9] and for labeling of bones in live animals.

References

  1. ^ Allen, T.M.; Cleland, L.G. (1980). "Serum-induced leakage of liposome contents". Biochimica et Biophysica Acta (BBA) - Biomembranes. 597 (2): 418–426. doi:10.1016/0005-2736(80)90118-2. PMID 7370258.
  2. ^ Sendai virus induced leakage of liposomes containing gangliosides Yung Shyeng Tsao and Leaf Huang Biochemistry 1985 24 (5), 1092-1098
  3. ^ Patel, H.; Tscheka, C.; Heerklotz, H. (2009). "Characterizing vesicle leakage by fluorescence lifetime measurements". Soft Matter. 5 (15): 2849–2851. Bibcode:2009SMat....5.2849P. doi:10.1039/B908524F.
  4. ^ "How Light Microscopes Work". HowStuffWorks. 25 May 2001.
  5. ^ Hamann, J.F.; Kiilgard; Litman, T.; Alvarez-Leefmans, J.; Zeuthen, T. (2002). "Measurement of cell volume changes by fluorescence self-quenching". J. Fluorescence. 12 (2): 139–145. doi:10.1023/a:1016832027325. S2CID 20539474.
  6. ^ "Fluorescent Indicators for Zn2+ and Other Metal Ions—Section 19.7". Molecular Probes: The Handbook. invitrogen.
  7. ^ Glavinas H, Krajcsi P, Cserepes J, Sarkadi B (January 2004). "The role of ABC transporters in drug resistance, metabolism and toxicity". Curr Drug Deliv. 1 (1): 27–42. doi:10.2174/1567201043480036. PMID 16305368. Archived from the original on 2012-07-19.
  8. ^ Karászi E, Jakab K, Homolya L, et al. (February 2001). "Calcein assay for multidrug resistance reliably predicts therapy response and survival rate in acute myeloid leukaemia". Br. J. Haematol. 112 (2): 308–14. doi:10.1046/j.1365-2141.2001.02554.x. PMID 11167823.
  9. ^ "Marking fry using calcein". Game & Wildlife Conservation Trust. Archived from the original on September 25, 2006.
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