HNRPU

HNRNPU
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1ZRJ
Identifiers
Aliases	HNRNPU, HNRPU, SAF-A, U21.1, hnRNP U, SAFA, HNRNPU-AS1, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), heterogeneous nuclear ribonucleoprotein U, HNRNPU antisense RNA 1, C1orf199, NCRNA00201, EIEE54, pp120, GRIP120, DEE54
External IDs	OMIM: 602869; MGI: 1858195; HomoloGene: 22991; GeneCards: HNRNPU; OMA:HNRNPU - orthologs
Gene location (Human) Chr. Chromosome 1 (human) Band 1q44 Start 244,840,638 bp End 244,864,560 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sperm ventricular zone ganglionic eminence tendon of biceps brachii caput epididymis Achilles tendon bronchial epithelial cell epithelium of colon trabecular bone corpus epididymis n/a More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nucleotide binding protein binding telomerase RNA binding RNA binding RNA polymerase II complex binding TFIIH-class transcription factor complex binding DNA binding chromatin binding double-stranded DNA binding single-stranded DNA binding transcription corepressor activity double-stranded RNA binding single-stranded RNA binding mRNA 3'-UTR binding actin binding poly(A) binding snRNA binding poly(C) RNA binding chromatin DNA binding poly(G) binding pre-mRNA binding ribonucleoprotein complex binding protein-containing complex binding sequence-specific double-stranded DNA binding ATP binding identical protein binding sequence-specific DNA binding RNA polymerase II C-terminal domain binding promoter-specific chromatin binding RNA polymerase II cis-regulatory region sequence-specific DNA binding Cellular component CRD-mediated mRNA stability complex cytoplasm catalytic step 2 spliceosome membrane nucleoplasm telomerase holoenzyme complex cytoplasmic ribonucleoprotein granule spliceosomal complex nucleus extracellular matrix nuclear chromosome cell surface nuclear matrix nuclear speck protein-containing complex RNA polymerase II transcription regulator complex inactive sex chromosome ribonucleoprotein complex kinetochore centrosome midbody mitotic spindle mitotic spindle midzone mitotic spindle microtubule chromosome, centromeric region spindle pole chromosome microtubule organizing center spindle cytoskeleton dendrite cytoplasm Biological process CRD-mediated mRNA stabilization mRNA splicing, via spliceosome RNA processing negative regulation of telomere maintenance via telomerase rhythmic process mRNA processing osteoblast differentiation RNA splicing RNA metabolic process positive regulation of gene expression circadian regulation of gene expression cardiac muscle cell development cellular response to dexamethasone stimulus negative regulation of transcription by RNA polymerase II regulation of alternative mRNA splicing, via spliceosome dosage compensation by inactivation of X chromosome negative regulation of kinase activity negative regulation of transcription elongation from RNA polymerase II promoter mRNA stabilization maintenance of protein location in nucleus cellular response to glucocorticoid stimulus RNA localization to chromatin positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity positive regulation of stem cell proliferation regulation of mitotic cell cycle positive regulation of transcription by RNA polymerase II regulation of mitotic spindle assembly regulation of chromatin organization positive regulation of attachment of mitotic spindle microtubules to kinetochore protein localization to spindle microtubule cellular response to leukemia inhibitory factor negative regulation of stem cell differentiation chromatin organization cell cycle multicellular organism development cell differentiation cell division positive regulation of brown fat cell differentiation dendritic transport of messenger ribonucleoprotein complex adaptive thermogenesis Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 3192 51810 Ensembl ENSG00000153187 ENSMUSG00000039630 UniProt Q00839 Q5RI18 Q8VEK3 RefSeq (mRNA) NM_004501 NM_031844 NM_016805 RefSeq (protein) NP_004492 NP_114032 NP_058085 Location (UCSC) Chr 1: 244.84 – 244.86 Mb n/a PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Heterogeneous nuclear ribonucleoprotein U is a protein that in humans is encoded by the HNRNPU gene.

Function

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that form complexes with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain. This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way. Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites. But this cleavage does not affect the function of the encoded protein in RNA metabolism. At least two alternatively spliced transcript variants have been identified for this gene.

Interactions

HNRPU has been shown to interact with:

• EP300,
• GTF2F1,
• Glucocorticoid receptor, and
• NDN.

Further reading

• Kiledjian M, Dreyfuss G (1992). "Primary structure and binding activity of the hnRNP U protein: binding RNA through RGG box". EMBO J. 11 (7): 2655–64. doi:10.1002/j.1460-2075.1992.tb05331.x. PMC 556741. PMID 1628625.
• Barel M, Balbo M, Gauffre A, Frade R (1995). "Binding sites of the Epstein-Barr virus and C3d receptor (CR2, CD21) for its three intracellular ligands, the p53 anti-oncoprotein, the p68 calcium binding protein and the nuclear p120 ribonucleoprotein". Mol. Immunol. 32 (6): 389–97. doi:10.1016/0161-5890(95)00005-Y. PMID 7753047.
• Sidorenko SP, Law CL, Chandran KA, Clark EA (1995). "Human spleen tyrosine kinase p72Syk associates with the Src-family kinase p53/56Lyn and a 120-kDa phosphoprotein". Proc. Natl. Acad. Sci. U.S.A. 92 (2): 359–63. doi:10.1073/pnas.92.2.359. PMC 42739. PMID 7831290.
• Jordan P, Heid H, Kinzel V, Kübler D (1995). "Major cell surface-located protein substrates of an ecto-protein kinase are homologs of known nuclear proteins". Biochemistry. 33 (49): 14696–706. doi:10.1021/bi00253a007. PMID 7993898.
• Liu Q, Dreyfuss G (1996). "A novel nuclear structure containing the survival of motor neurons protein". EMBO J. 15 (14): 3555–65. doi:10.1002/j.1460-2075.1996.tb00725.x. PMC 451956. PMID 8670859.
• Fujioka Y, Matozaki T, Noguchi T, Iwamatsu A, Yamao T, Takahashi N, Tsuda M, Takada T, Kasuga M (1997). "A novel membrane glycoprotein, SHPS-1, that binds the SH2-domain-containing protein tyrosine phosphatase SHP-2 in response to mitogens and cell adhesion". Mol. Cell. Biol. 16 (12): 6887–99. doi:10.1128/MCB.16.12.6887. PMC 231692. PMID 8943344.
• Dennehy KM, Broszeit R, Garnett D, Durrheim GA, Spruyt LL, Beyers AD (1997). "Thymocyte activation induces the association of phosphatidylinositol 3-kinase and pp120 with CD5". Eur. J. Immunol. 27 (3): 679–86. doi:10.1002/eji.1830270316. PMID 9079809.
• Malik KF, Jaffe H, Brady J, Young WS (1997). "The class III POU factor Brn-4 interacts with other class III POU factors and the heterogeneous nuclear ribonucleoprotein U." Brain Res. Mol. Brain Res. 45 (1): 99–107. doi:10.1016/S0169-328X(96)00238-0. PMID 9105675.
• Eggert M, Michel J, Schneider S, Bornfleth H, Baniahmad A, Fackelmayer FO, Schmidt S, Renkawitz R (1997). "The glucocorticoid receptor is associated with the RNA-binding nuclear matrix protein hnRNP U." J. Biol. Chem. 272 (45): 28471–8. doi:10.1074/jbc.272.45.28471. PMID 9353307.
• Göhring F, Schwab BL, Nicotera P, Leist M, Fackelmayer FO (1998). "The novel SAR-binding domain of scaffold attachment factor A (SAF-A) is a target in apoptotic nuclear breakdown". EMBO J. 16 (24): 7361–71. doi:10.1093/emboj/16.24.7361. PMC 1170336. PMID 9405365.
• Hahm B, Cho OH, Kim JE, Kim YK, Kim JH, Oh YL, Jang SK (1998). "Polypyrimidine tract-binding protein interacts with HnRNP L.". FEBS Lett. 425 (3): 401–6. doi:10.1016/S0014-5793(98)00269-5. PMID 9563502.
• Matsui M, Breau WC, Iwasaki S, Hagiwara S, Tamai Y, Mori C, Bloom ML, Jerry MB, Eddy EM, Taketo MM (1999). "Retrovirus integration site Mintb encoding the mouse homolog of hnRNP U.". J. Biochem. 125 (6): 1104–14. doi:10.1093/oxfordjournals.jbchem.a022392. PMID 10348913.
• Kim MK, Nikodem VM (2000). "hnRNP U inhibits carboxy-terminal domain phosphorylation by TFIIH and represses RNA polymerase II elongation". Mol. Cell. Biol. 19 (10): 6833–44. doi:10.1128/MCB.19.10.6833. PMC 84680. PMID 10490622.
• Kipp M, Schwab BL, Przybylski M, Nicotera P, Fackelmayer FO (2000). "Apoptotic cleavage of scaffold attachment factor A (SAF-A) by caspase-3 occurs at a noncanonical cleavage site". J. Biol. Chem. 275 (7): 5031–6. doi:10.1074/jbc.275.7.5031. PMID 10671544.
• Husi H, Ward MA, Choudhary JS, Blackstock WP, Grant SG (2000). "Proteomic analysis of NMDA receptor-adhesion protein signaling complexes". Nat. Neurosci. 3 (7): 661–9. doi:10.1038/76615. hdl:1842/742. PMID 10862698.
• Taniura H, Yoshikawa K (2002). "Necdin interacts with the ribonucleoprotein hnRNP U in the nuclear matrix". J. Cell. Biochem. 84 (3): 545–55. doi:10.1002/jcb.10047. PMID 11813259.
• Lee J, Bedford MT (2002). "PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays". EMBO Rep. 3 (3): 268–73. doi:10.1093/embo-reports/kvf052. PMC 1084016. PMID 11850402.
• Davis M, Hatzubai A, Andersen JS, Ben-Shushan E, Fisher GZ, Yaron A, Bauskin A, Mercurio F, Mann M, Ben-Neriah Y (2002). "Pseudosubstrate regulation of the SCF(beta-TrCP) ubiquitin ligase by hnRNP-U". Genes Dev. 16 (4): 439–51. doi:10.1101/gad.218702. PMC 155337. PMID 11850407.